摘要
目的研究肝癌细胞和白血病细胞诱导分化时α１，６岩藻糖转移酶（α１，６ＦｕｃＴ）的改变。方法采用生物素标记受体糖链，用ＬＣＡ亲和层析柱分离被核心岩藻糖化的产物，并以辣根过氧化酶（ＨＲＰ）标记的亲和素与柱上的产物结合，最后测定洗脱液中ＨＲＰ的活力，可代表α１，６ＦｕｃＴ活力。结果全反式维甲酸（ＡＴＲＡ）或双丁酰环磷腺苷（ｄｂ－ｃＡＭＰ）诱导７７２１人肝癌细胞分化后，α１，６－ＦｕｃＴ活力均降低。ＡＴＲＡ或正丁酸使ＨＬ－６０早幼粒白细胞分化成粒细胞，佛波醇－１２－豆蔻酸－１３乙酸酯（ＰＭＡ）使ＨＬ－６０细胞分化成单核细胞时都使α１，６ＦｕｃＴ活力下降，而用二甲亚砜（ＤＭＳＯ）或神经节苷酯ＧＭ３使ＨＬ－６０细胞分化成粒细胞或单核细胞时，α１，６ＦｕｃＴ活力则上升。结论α１，６－ＦｕｃＴ活力下降是肝癌细胞的分化指标，但和ＨＬ－６０细胞的分化关系不大。
Purpose To study the changes of the activity α1,6fucosyltransferase(α1,6FucT) during the induced differentiation of hepatocarcinoma cells and leukemia cells. Methods Biotinlabeled acceptor Nglycan was used as substrate.The corefucosylated product was isolated from substrate using LCA lectin affinity chromatographic column.After HRPavidin conjugate was bound to the biotinylated product on the column,the HRP avidin biotinylated product was eluted from the column and the activity of HRP was determined as a measure of α 1,6FucT activity. Results α 1,6FucT activity was decreased in the induced differentiation of human hepatocarcinoma cell line 7721 by both ATRA and db cAMP.α1,6FucT was also decreased during the induced differentiation of HL60 cells to myelocyte by ATRA or n butysic acid,as well as to monocyte by PMA.Whereas,the enzyme activity was increased when HL60 cells were induced to differentiate to myelocyte by DMSO and to monocyte by ganglioside GM 3. Conclusions The decrease of α 1,6FucT activity is an index of differentiation for hepatocarcinoma cells,but not for HL60 cells.
出处
《上海医科大学学报》
CSCD
1999年第2期122-124,共3页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金
关键词
α-1
6-FucT
肝癌细胞
HL-60
白血病细胞
诱导
α 1
6fucosyltransferase
7721 human hepatocarcinoma cells
HL60 premyelocytic leukemia cell
induced differentiation
