摘要
利用RNA干扰原理可以对作物进行遗传改良。构建RNA干扰的发夹结构一般采用在引物两端加酶切位点方法,该方法涉及酶切位点多,步骤繁琐。本试验介绍一种直接利用PCR产物进行RNAi组成型表达载体构建的方法,该载体以潮霉素为筛选标记基因,适合水稻的遗传转化。
RNAi has been widely used in plant genetic improvement and gene function analysis in recent years. The conventional way to construct the hairpin structure of RNAi binary vector is based on the restriction enzyme digestion and ligation. However,this method is labour intensive and time consumption. In this study,we established a binary transgenic vector with PCR product,which habouring Hpt resistant gene and driving by constitutive promoter,and which would facilitate further rice transformation.
出处
《华北农学报》
CSCD
北大核心
2010年第5期52-55,共4页
Acta Agriculturae Boreali-Sinica
基金
国家转基因生物新品种培育科技重大专项(2009ZX08001-005B
019)
江苏省自然科学基金项目(BK2009321)
国家科技支撑计划重大项目(2006BADO2A03)
江苏省农业科技自主创新基金项目(CX[07]603)
江苏省政府留学奖学金
江苏省农业科学院基金项目(6110703
6510806)
