摘要
为了验证苹果多聚半乳糖醛酸酶抑制蛋白(PGIP)基因表达产物对植物病原真菌的作用,获得转基因植株,培育抗病新品种,本研究用限制性内切酶SalⅠ、BamHⅠ将已克隆的苹果PGIP基因从克隆载体pMD-18T上切下,定向插入到植物表达载体pWR306的ED35s启动子和TNOS终止子中间,成功构建了苹果PGIP基因植物表达载体pWR306-PGIP及工程农杆菌,以番茄中蔬四号叶片及茎段为受体,通过农杆菌介导法转化,获得了8株PCR检测阳性的番茄植株。
Aimed to study the function of polygalacturonase-inhibiting protein gene in plant,breed disease resistant cultivar,obtain the transgenic plant,a plant expression vector pWR306-PGIP was constructed by inserting the PGIP gene coding fragment from cloning vector pMD-18T by SalⅠand BamHⅠsites into pWR306 between of ED35s promotor and NOS terminator. The identification results showed that the plant expression vector pWR306-PGIP and Agrobacterium tumefaciems were constructed successfully. Subsequently PGIP gene was transformed into Zhongshu No. 4 tomato leaves and shoot fragments mediated by Agrobacterium tumerfaciens. Eight transgenic resistant plants were proved by PCR preliminarily that the PGIP gene had integrated into tomato genome.
出处
《华北农学报》
CSCD
北大核心
2010年第5期42-46,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(30671447)
