摘要
目的研究不同强度的磁性附着体静磁场对成骨细胞矿化能力的影响。方法对体外培养的SD大鼠成骨细胞分别进行12.5、125、250mT的静磁场加载1、3、5、7d,考马斯亮篮法测定碱性磷酸酶(ALP)活性;静磁场加载21d后,茜素红S钙染法检测矿化结节并计数。结果静磁场加载1d后,各磁场处理组和对照组相比,ALP活性无明显差异(P>0.05)。静磁场加载3d后,125mT和250mT磁场组ALP活性和对照组差异有统计学意义(P<0.05);静磁场加载5、7d后,各磁场组ALP活性与对照组差异有统计学意义(P<0.01),而且125mT和250mT磁场组ALP活性高于12.5mT磁场组。静磁场加载21d后,各磁场组矿化结节计数高于对照组。结论在本实验条件下,磁性附着体静磁场可以促进成骨细胞的ALP活性和矿化能力。
Objective To study the effects of the various static magnetic field （SMF） intensity of the magnetic attachment system on the alkaline phosphatase （ALP） activity and the mineralization capability of the osteoblast. Methods The in vitro cultured SD rat osteoblasts were exposed continuously to 12.5 mT,125 mT,and 250 mT SMF for 1,3,5,and 7 days in the test groups,while the control group without SMF exposure were used for comparison. The activity of cell ALP was measured with a spectrophotometer and the capability of mineralization was measured by alizarin red S （ARS） staining. Statistical analysis was performed. Results For 1-d SMF exposure,there was no statistical significance （P 0.05） in the ALP activity of osteoblast among all the testing groups and the control groups. For 3-d SMF exposure,statistical significances （P 0.05） were found between the 125,250 mT group and the control group. When the SMF exposure time was extended to 5 and 7 days,the ALP activities of all the testing groups showed significant differences from the control group （P 0.01）. In addition,the ALP activities of the 125 mT and 250 mT groups were higher than that of the 12.5 mT group. The number of the mineral nodes of the three exposed groups was significantly larger than that of the control group. Conclusions Static magnetic field of magnetic attachment can stimulate the ALP activity and the mineralization capability of the osteoblast under the conditions defined in this study.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2010年第5期29-32,共4页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
广东省自然科学基金博士启动项目(9451008901002644)
关键词
磁性附着体
静磁场
成骨细胞
碱性磷酸酶
Magnetic attachments Static magnetic fields Osteoblast Alkaline phosphatase
