摘要
Almost all clinically important RBC antigens are defined at the molecular level.The expression of protein-and sugar-based antigens on the RBC surface can be predicted by determining the blood group gene variants (alleles).Most of the time,a single nucleotide polymorphism (SNP) distinguishes the allele,which determines an antigen and hence allows predicting the antigen.PCR with sequence specific priming (PCR-SSP) followed by gel electrophoresis was the original technique widely applied for blood group genotyping.Real-time PCR obviated the need for gels.The proof of principle for high-throughput platforms was a hybridization based DNA chip,in quick succession followed by Beadchip,SNPstream,SNaPshot and Luminex technology.Each of these platforms differs in technical details,which can put one of them at an advantage over the others depending on the specific tasks and needs that it is expected to cover.
Almost all clinically important RBC antigens are defined at the molecular level.The expression of protein-and sugar-based antigens on the RBC surface can be predicted by determining the blood group gene variants （alleles）.Most of the time,a single nucleotide polymorphism （SNP） distinguishes the allele,which determines an antigen and hence allows predicting the antigen.PCR with sequence specific priming （PCR-SSP） followed by gel electrophoresis was the original technique widely applied for blood group genotyping.Real-time PCR obviated the need for gels.The proof of principle for high-throughput platforms was a hybridization based DNA chip,in quick succession followed by Beadchip,SNPstream,SNaPshot and Luminex technology.Each of these platforms differs in technical details,which can put one of them at an advantage over the others depending on the specific tasks and needs that it is expected to cover.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2010年第10期816-816,共1页
Chinese Journal of Blood Transfusion
