摘要
目的探讨Alzheimer病(AD)患者血浆中微管相关蛋白(Tau protein,Tau蛋白)与载脂蛋白 E(apolipoproteinE,ApoE)多态性的关系。方法采集92例AD患者及100名同龄健康老年人的外周血,采取聚合酶链式反应-限制性片段长度多态性法(PCR-RFLP)进行ApoE基因多态性分析,同时应用酶联免疫吸附剂测定法(ELISA)对血浆中Tau蛋白进行定量分析。结果与对照组[(5.2±4.1)pg/mL]相比,AID患者血浆中Tau蛋白水平[(1 5.4±7.9)pg/mL]升高(P<0.01),携带ApoEε4等位基因的AD患者Tau蛋白水平[(21.3±4.6)pg/mL]升高更明显(P<0.01)。结论 AD患者血浆中Tau蛋白水平与ApoEε4等位基因相关,有可能作为早期AD诊断的生物学指标。
Objective To study the relationship between plasma Tau protein and apolipoprotein E （ApoE） gene types in Alzheimer disease （AD） . Methods The peripheral blood of 92 AD patients and 100 healthy controls were selected. The polymerase chain reaction-restriction fragment length polymorphism method （PCR-RFLP） was used for ApoE gene polymorphism analysis, and enzyme linked immunosorbent assay method （ELISA） was selected for quantitative analysis of Tau protein. Results Compared with healthy controls f （5.2 ±4.1） pg/mL], Tau protein concentration in plasma increased in AD patients [ （21.3±4.6） pg/mL, P〈 0.01）, especially in those carrying ApoEε4 allele [（21.3±4.6） pg/mL, P〈0. 013 . Conclusions Tau protein concentration is associated with ApoEε4, and it may be used as the biological indicators of early AD diagnosis.
出处
《中国神经免疫学和神经病学杂志》
CAS
2010年第6期405-407,共3页
Chinese Journal of Neuroimmunology and Neurology
基金
吴阶平医学基金会临床科研专项资助基金(320.6750.08183)
