摘要
目的研究细胞毒素相关抗原（ｃａｇＡ）基因在幽门螺杆菌（Ｈｐ）菌株中的分布，从而明确中国人感染Ｈｐ菌株的毒力状况。方法采用特异性引物扩增ＨｐｃａｇＡ基因的２９７ｂｐ片段，对７４个临床分离Ｈｐ菌株采用聚合酶链反应（ＰＣＲ）进行分型。同时收集患者的胃镜诊断及胃窦病理资料。结果９０．５％的Ｈｐ菌株含有ｃａｇＡ基因，其中消化性溃疡（ＰＵ）患者感染菌株ｃａｇＡ基因携带率（９４．９％）高于慢性胃炎患者（８５．７％），但二者差异无显著性（Ｐ＞０．０５）。病理资料显示，Ⅰ、Ⅱ型菌株均可引起胃窦的慢性炎症，但严重程度Ⅱ型菌株（ｃａｇＡ－）高于Ⅰ型（ｃａｇＡ＋，Ｐ＜０．０５），二者在致活动性胃炎、肠上皮化生、胃粘膜萎缩及淋巴滤泡形成方面比较，差异无显著性（Ｐ＞０．０５）。结论中国人感染ＨｐⅠ型菌株比例较西方国家高，但ｃａｇＡ基因尚不能作为区分Ｈｐ感染致不同胃肠道疾病的单一指标。
Objective To study the distribution of strains and the virulence of H.pylori affecting Chinese patients. Methods Hp pattern was determined by PCR in 74 clinical isolates by using one primer set for the cagA gene. Results The prevalence of cagA gene in Hp isolates was 90.5% and the positive rate of cagA gene in peptic ulcer patients was 94.9%, which was higher than that in chronic gastritis patients (85.7%), but there was no significant statistical difference. Both type (cagA+) and type (cagA-) isolates could lead to the chronic inflammation of the antrum, but the inflammation caused by type was more severe than that by type . There were no significant differences in severity of active gastritis, atrophy, intestinal metaplasia, and lymph follicles. Conclusion The high prevalence of the cagA gene in H.pylori isolates among Chinese patients indicates that the cagA gene is more common than that among westernese, but it is not reliable as a single marker for discrimination of H.pylori strains with respect to a specific gastric disease.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1999年第3期165-167,共3页
Chinese Journal of Digestion
基金
国家自然科学基金
