摘要
采用表面聚合法,以抗蚜威为模板分子,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,选择不同的功能单体,通过硅胶表面接枝偶氮引发剂在庚烷及乙腈溶剂中引发热聚合反应,制备得到不同的分子印迹聚合物(MIP)。将MIP应用于固相萃取(SPE)柱填料,并通过高效液相色谱(HPLC)法比较各种聚合物的吸附性能。结果表明以三氟甲基丙烯(TFMAA)为功能单体、庚烷为反应溶剂制备得到的MIP具有较好的选择性吸附效果:选择性系数为8.60,相对选择性系数为7.29。以不同溶剂对该MIP上样,推断聚合物与抗蚜威之间主要通过氢键作用结合,在水介质中还存在静电相互吸引作用。通过与市售C18填料的SPE柱进一步比较,证明了该MIP具有良好的特异性吸附性能,这将为完善以MIP为填料的SPE-HPLC法痕量检测果蔬中抗蚜威提供了参考和依据。
On the theoretical basis of surface polymerization, different kinds of molecularly imprinted polymers based on modified silica gel were prepared. The thermal polymerization was initiated by azo-initiator grafted on silica surface in heptane or aeetonitfile using pirimicarb as template and EDMA as erosslinker, and choosing different functional monomers. The MIPs obtained were used in solid-phase extraction （SPE） and detected the specific： absorption property by high performance liquid chromatography （HPLC）. The results showed that TFMAA and heptance were necessary for the MIP with high-pertbrmanee recognition with 8.60 of the selectivity coefficient in aqueous solution, and 7.29 of the relative selective coefficient compared to NIP. It was deduced from different loading solutions results that the polymers and pirimicarb mainly could combine through tlydrogen bond. Besides, there was still being electrostatic interaction between MIP and pirimicarb in aqueous solution. The MIP had good specific adsorption and separation of pirimicarb in further comparison with C18, thus this study will provide reference and basis for the further improvement of trace detection for pirimicarb in fruits and vegetables by MIP-SPE-HPLC.
出处
《上海交通大学学报（农业科学版）》
2010年第3期286-291,共6页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科委标准化项目子课题(07DZ05019)
关键词
分子印迹聚合物
抗蚜威
固相萃取-高效液相色谱法
molecularly imprinted polymers, pirimicarb
solid phase extraction-high performance liquid chromatography （SPE-HPLC）
