摘要
通过研究添加不同的浓度肝素(0、5、10、20、50μg/mL)及不同作用时间(0、30、45、60、90、120min)对绵羊精子体外获能、活力、存活时间和相应体外受精的影响,为改善绵羊精子体外获能效果及研究获能的机理提供一定的参考。在获能液中添加10μg/mL肝素作用45min时,其获能率最高,顶体反应率最低(40.92%和16.59%)(P<0.05),存活时间18h,能够提高受精卵的囊胚发育;单独使用咖啡因或与10μg/mL肝素联合使用,其获能效果均不如单独使用10μg/mL肝素。说明绵羊精子获能时肝素的适宜浓度是10μg/mL,作用时间是45min,咖啡因无协同肝素促进获能的作用。
In order to find out some modified methods of ovine sperm capacitation in vitro,the effects of their sperm capacitation,motility,enduring durations and fertilization after respectively adding with 0,5,10,20,50 μg/mL heparin at different time （0,30,45,60,90,120 min）,were compared and analyzed.The results showed that ovine sperm capacitation rate was highest （40.92%）,acrosome reaction was lowest （16.59%）,enduring durations were longest （18 h）,and the blastula rate was highest,when adding with 10 μg/mL heparin in the capacitation medium for 45 min.The effects of capacitation when adding with caffeine or caffeine and 10 μg/mL heparin,were not as good as the effects adding with 10 μg/mL heparin alone.It can be recommended that it is applicable for improving ovine capacitation in vitro system with 10 μg/mL heparin for 45 min,caffeine don ＇ t cooperate with heparin to improve the rate of capacitation.
出处
《中国农学通报》
CSCD
北大核心
2010年第21期21-24,共4页
Chinese Agricultural Science Bulletin
基金
国家"十一五""863计划"重点项目"家畜卵泡高效诱导发育技术研究与应用"(2008AA101003)
关键词
绵羊精子
体外获能
肝素
咖啡因
体外受精
ovine sperm
capacitation in vitro
heparin
caffeine
fertilization in vitro
