摘要
目的构建hSAMP32避孕疫苗,研究其对小鼠生育率的影响。方法扩增hSAMP32基因片段;hSAMP32基因测序;重组质粒pcDNA3.1(+)-hSAMP32的构建;观察BALB/c小鼠生殖能力是否受到影响。结果经对比,目的基因组较对照组穿卵率明显下降。结论 hSAMP32基因可以降低BALB/c小鼠的生殖能力。
Objective To construct hSAMP32 contraceptive vaccine, and to study its impact on fertility in BALB/c mice. Methods The authors amplified gene hSAMP32, sequeuced hSAMP32 gene, reconstructed DNA vaccine（pcDNA3.1 （ ＋ ）-hSAMP32）, using gene-gun to put DNA vaccine into BALB/c mice, and observed the BALB/c mice＇ s reproductive ability. Results The gravidity rate decreased greatly compared to the control group. Conclusion hSAMP32 can be used as immunocontraceptive vaccine to decrease the rate of pregnancy of mouse.
出处
《河南职工医学院学报》
2010年第6期637-639,共3页
Journal of Henan Medical College For Staff and Workers
