摘要
通过以体质量６０～７０ｇ的幼年鲤鱼（Ｃｙｐｒｉｎｕｓｃａｒｐｉｏ），每ｋｇ体质量以２００ｍｇ的剂量体腔注射链脲佐菌素（ｓｔｒｅｐｔｏｚｏｔｏｃｉｎ，ＳＴＲ）或载体溶液２００ｍｇ，观察鲤鱼生长、血糖、血清生长激素（ＧＨ）水平和肝组织胰岛素样生长因子Ⅰ（ＩＧＦⅠ）ｍＲＮＡ水平的变化．注射ＳＴＲ的鱼生长速率比对照组显著减慢．在第１５天和第３０天实验组鲤鱼血糖水平以及血清ＧＨ水平皆无明显变化．在注射ＳＴＲ后的第１５天肝组织ＩＧＦⅠｍＲＮＡ的表达水平比对照组明显下降，第３０天两组鱼肝组织ＩＧＦⅠｍＲＮＡ含量则无明显差异．注射ＳＴＲ后鲤鱼出现胰岛遭受破坏的症状．实验结果提示ＳＴＲ可能通过破坏胰岛Ｂ细胞导致胰岛素（Ｉｎｓ）分泌下降，而后者则可能通过ＧＨ受体水平或ＧＨ受体以后水平的某种机制导致鲤鱼肝组织ＩＧＦⅠｍＲＮＡ表达的下降。
Juvenile common carp, about 60～70 g, were injected intraperitoneally either with 200 mg/kg body wt STR, an agent known to induce damage of pancreatic B cells in mammals, or with the vehicle in order to study the effect of STR on the growth and hepatic IGF ⅠmRNA expression in common carp The control fish grew quickly throughout the experiment, while the growth of STR injected fish was retarded There was no difference between the two groups of fish in plasma levels of either glucose or growth hormone In corresponding with the fish growth state, on the day 15 post STR injection, the hepatic IGF ⅠmRNA level declined significantly On the day 30, the hepatic IGF ⅠmRNA level of STR injected fish was somehow restored These results imply STR injection may induce hypoinsulinemia by causing pancreatic B cells destruction to some extent and then lead to diminished hepatic IGF Ⅰ production, which eventually lead to the growth retardation of STR treated common carp
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第3期72-75,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
中山大学基础性研究前沿基金
