摘要
从病毒感染后期的斜纹夜蛾幼虫脂肪体中提取ｍＲＮＡ，反转录合成ｃＤＮＡ探针，并使之与ＳｌＮＰＶ基因组的酶切片段杂交，将ＳｌＮＰＶｐ１０基因定位于ＢａｍＨⅠ３０千碱基片段上．ＳｌＮＰＶｐ１０是目前发现的最长ｐ１０基因；基因的启动子内含有２个ＡＴＴＧＴＡ基序，是目前所发现的ｐ１０基因中唯一含有２个Ａ／ＴＴＴＧＴＡ基序者．ＳｌＮＰＶＰ１０蛋白含有１０个七肽重复单元，可形成１个相对较长的卷曲螺旋．ＳｌＮＰＶｐ１０所在的ＯＲＦ５５２ｐ１０ＯＲＦ９４５基因簇与ＳｐｌｉＮＰＶ的ＯＲＦ５５２ｐ１０ＯＲＦ９４５基因簇各基因座位、方向及对应的氨基酸序列十分相似。
The p 10 gene was localized within the 3 0 kb Bam HⅠ fragment of the Sl NPV genome by Southern hybridization. With a coding sequence of 318 nucleotides, corresponding to a protein of 105 amino acids, the p 10 gene of Sl NPV is the longest p 10 gene identified so far in NPVs. The Sl NPV p 10 gene is also distinct in having two A/TTTGTA motifs in the promoter region. With 10 heptad repeats, the Sl NPV P10 protein is probably forming a relatively large coiled coil structure. The organization of Sl NPV ORF552 p 10 gene ORF945 cluster is colinear with that of Spli NPV and their corresponding amino acid sequences of each ORF share high similarity. This indicates that Sl NPV and Spli NPV belongs to a homologous baculovirus group.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第3期57-62,共6页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金
