摘要
目的观察中草药桔梗对丝裂霉素(MMC)诱发小鼠微核的抑制作用。方法 50只小鼠随机分为阴性对照组(N·S),阳性对照组(MMC,30mg·kg-1);桔梗水煎液诱变组(1.0、2.0、4.0和8.0g·kg-1,相当于人5、10、20、40倍临床常用剂量);桔梗水煎液抗诱变组(MMC0.4mg·kg-1+桔梗各剂量组)。每组5只动物,雌雄兼用。MMC腹腔注射给药,其他药物均为灌胃给药,抗诱变实验组桔梗和MMC同时给药,小鼠骨髓嗜多染红细胞微核实验按常规方法进行。结果桔梗各剂量组所诱导的小鼠微核频率与正常对照组比较,差异无显著性(P>0.05)。桔梗本身不能诱发小鼠微核,并且4.0g·kg-1,8.0g·kg-1组与丝裂霉素一同给药时,可使丝裂霉素所诱发的小鼠微核率明显降低(P<0.05)。结论桔梗对遗传物质无诱变作用,对小鼠遗传物质具有一定的保护作用。
Objective To observe the inhibitory effects of Platycodon grandiflorum（Jaoq.） A.DC on micronuclei frequencies induced by MMC in the bone marrow cells of mice.Methods Fifty mice were divided into groups： negative control （N·S）,positive control（MMC 0.4 mg·kg-1）,Platycodon grandiflorum（Jaoq.）A.DC mutagenesis groups（1.0、2.0、4.0 and 8.0g·kg-1,which were matched 5、10、20、40 clinical commonly used dosage of a person）Platycodon grandiflorum（Jaoq.）A.DC antimutagenesis groups（MMC 30mg·kg-1＋each Platycodon grandiflorum（Jaoq.）A.DC dosage groups）,5 mice per groups.MMC are given by drug abdominal cavity Injection,other drug are given by perfusing stomach.Platycodon grandiflorum（Jaoq.）A.DC and MMC are given simultaneously in antimutagenesis groups,once a day for 5 days successively.At the 5th weekend after the first administration,the mice are killed by cervical vertebra dislocation whose marrow are drawed and sheets are produced in our laboratory method.Results the micro-nuclei frequencies was no significant difference between tested groups and control group（P0.05）,but the frequency of micronucle induced by MMC was reduced by intragastrial perfusion of Platycodon grandiflorum（Jaoq.）A.DC（4.0g·kg-1,8.0g·kg-1） and MMC at the same time（P0.01）.Conclusion Platycodon grandiflorum（Jaoq.）A.DC can not induce micronulei formation in the mouse bone marrow cells,but shows a protective effect on genetic materials in mice.
出处
《中国药物警戒》
2010年第11期644-645,共2页
Chinese Journal of Pharmacovigilance
关键词
桔梗
丝裂霉素
抗诱变
Platycodon grandiflorum（Jaoq.）A.DC
mitomycin
antimutagenic agents
