摘要
目的:观察黄药子乙醇提取物(ET)的抗炎活性。方法:①采用小鼠二甲苯致耳肿胀炎症模型,考察ET(50、100、200mg/kg)的抗炎活性,并考察了在二甲苯诱导的小鼠炎症病理状态下ET对肝功能的影响;②通过分析比较耳内前列腺素PGE2含量,初步探讨了其抗炎机制。结果:①与模型组比较,ET(100、200mg/kg)组小鼠耳肿胀明显降低(P<0.05),ET(50mg/kg)有降低趋势(P>0.05),ET(50、100、200mg/kg)呈现出良好的量效关系;与阳性药阿司匹林(Aspirin,200mg/kg)比较,ET(200mg/kg)组小鼠耳肿胀无明显差异(P>0.05);ET(200mg/kg)对二甲苯诱导的炎症病理状态下小鼠血清ALT和AST活力均无影响(P>0.05),而Aspirin(200mg/kg)则可引起ALT和AST明显升高(P<0.05);②ET(200mg/kg)可明显降低小鼠炎症耳组织内过高的炎症因子PGE2含量(P<0.05)。结论:ET在100、200mg/kg对二甲苯诱发的小鼠急性炎症模型有效,并在同一剂量(200mg/kg)下与Aspirin抗炎作用相当,且避免了Aspirin用药诱发肝毒性的不良反应;ET抗炎作用机制可能与抑制炎症组织内炎症因子PGE2合成有关。
Objective ： To investigate the anti - inflammatory activity of the ethanol extract of Dioscorea bulbifera L. rhizome （ET）. Methods ： ①Ear edema induced by dimethylbenzene in mice was applied for evaluating the anti - inflammatory effect of ET, and then serum biomarkers including alanine transaminase （ALT） and aspartate transanimase （AST） for liver injury were also assayed in such mice. ②The anti - inflammatory mechanism was explored by measuring the content of PGE2 in mice edema ear. Results ： （~）Compared with Control, ET （ 100,200 mg/kg） significantly reduced the swelling degree of tumefaction in mice with auricle induced by dimethyl- benzene with no conspicuous difference in serum biomarkers for liver injury. Compared with the positive drug aspirin （200 mg/kg）, there is no significant difference in such swelling degree of ET （200 mg/kg）.②ET significantly reduced the content of PGE2 in mice edema ear. Conclusion ： ET has anti - inflammatory effect at doses of 100 and 200 mg/kg and the mechanism may be associated with reduced content of PGE2 in mice edema ear. Moreover, ET has not only an equivalent anti - inflammatory effect with aspirin at the same dose of 200 mg/kg, but also is avoid of the hepatotoxicity induced by aspirin.
出处
《中医学报》
CAS
2010年第6期1127-1129,共3页
Acta Chinese Medicine
基金
国家重点基础研究发展计划(973计划)(编号:2006CB504704)
国家自然科学基金(编号:30701082)
上海市自然科学基金(编号:09ZR1431500)
上海市科委科研计划项目(编号:08DZ1972300)
上海市教委科研创新项目(编号:09ZZ125)
关键词
黄药子
乙醇提取物
抗炎活性
小鼠
Dioscorea bulbifera L.
ethanol extract
anti - inflammatory activity
rat
