摘要
蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）是一类分布广泛的丝氨酸／苏氨酸蛋白激酶，在真核细胞的跨膜信号传递中发挥重要的作用［１］．而细胞周期进程是一个复杂的调控过程，受控于多种磷酸酶与磷酸激酶作用下的中心调控体系［２］．大量报道表明ＰＫＣ信号...
By using N 2O synchronized HeLa cells as a model, the effect of PKC activation and inhibition on HeLa cell G 1 to S phase progression was investigated. It was found that this process was remarkably influenced by TPA and GF 109203X treatment, which was the activator and inhibitor of PKC respectively. TPA (100 μg/L) could promote G 1/S progression; on the contrary, it was inhibited by GF 109203X (4 mg/L) treatment. Further study discovered this change was related to PKC activity. TPA treatment promoted PKC activity by 90 0%, and GF 109203X inhibited it by 74 1%. It was shown that MAPK signaling pathway also functioned in this process, which itself might be regulated by PKC activity. In conclusion, it was found that these two signaling pathways cooperated to regulate the progression from G 1to S phase.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第3期495-497,共3页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金
