摘要
人类免疫缺陷病毒Ⅱ型（ＨＩＶ－２）的跨膜蛋白ｇｐ３６，是从外膜蛋白前体中裂解的，它在诱导病毒与细胞膜的融合和合胞体的形成中起着重要的作用〔１〕。感染ＨＩＶ－１或ＨＩＶ－２的患者，可产生针对病毒结构蛋白（包括包膜表面糖蛋白和跨膜蛋白）的体液免疫〔２－４...
The human immunodeficiency virus type 2 (HIV-2) gp36 DNA fragment was
amplified by polymerase chain reaction from the HIV-2 ROD 2 cDNA template using a pair of the
designed primers containing Nde Ⅰ and Xho Ⅰ sites, then was ligated with pGEM-T vector by
T4 DNA ligase to construct the plasmid pGEMT-36. The gp36 fragment cutted with NdeⅠ and
XhoⅠwas inserted into the expression plasmid pExSecⅠincluding these two endonuclease
sites for constructing the recombinant plasmid pExSec Ⅰ-36. It was confirmed by PCR,
restrcition enzyme and sequence analysis. The recombinant protein was expressed in E. coli
, rgp36 was induced by IPTG to produce the recombinant protein, its molecular weight and
antigenicity were confirmed by the analysis of SDS-PAGE and Western blot.
出处
《病毒学报》
CAS
CSCD
北大核心
1999年第2期188-191,共4页
Chinese Journal of Virology
基金
国家攻关项目
关键词
外膜蛋白
原核表达
HIV-2
Human immunodeficiency virus type 2, Gp36, Prokaryotic expression
