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大鼠OB基因克隆及其在大肠杆菌中的表达 被引量:1

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摘要 采用RTPCR技术扩增大鼠OBcDNA编码区序列。PCR产物酶切后定向克隆至pUC19质粒。经核苷酸序列测定表明与文献报道的大鼠OBcDNA编码区序列一致。继之构建了pBV220rOB表达质粒并获得了OB基因在大肠杆菌中的特异表达。大鼠OB基因产物的获得为研究肥胖与某些非传染性疾病(如糖尿病。
出处 《微生物学报》 CAS CSCD 北大核心 1999年第3期215-219,共5页 Acta Microbiologica Sinica
基金 国家自然科学基金
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参考文献2

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同被引文献15

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