摘要
对聚β－羟基丁酸（ＰＨＢ）产生菌Ｚ５－ＧⅡ的发酵培养基及发酵条件进行优化研究；结果表明：该菌株在蔗糖１％，酵母粉０．３％，酵母浸汁０．３％，Ｋ２ＨＰＯ４０．２％；ｐＨ７．２～７．４的优化发酵培养基中，接种量８％，种龄２８ｈ，发酵培养３６ｈ，细胞干重为６．８７ｇ／Ｌ，ＰＨＢ产率可达的细胞干重的６１．８６％。该菌株还可利用葡萄糖生产废液为碳源生产ＰＨＢ，具有实现工业化生产的潜力。
The conditions for the production of poly β hydroxybutyric acid (PHB) by Zo ogloea , sp. (Z 5 GⅡ ) were optimized. The optimal condition s for PHB production were as follows: 28 h of culture peroid, 8% of inoculum con c entration, 36 h of fermentation period and the optimized medium containing 1% su c rose; 0.3% yeast extract; 0.3% yeast powder; 0.2% K 2HPO 4; pH 7.2￣7.4. The ma ximum content of PHB was up to 61.86% with 6.87 g/L of dry cell weight. The stra in also could use waste fluid from production of glucose as the carbon source to produce PHB. The strain had potential for the mass production of PHB.
出处
《工业微生物》
CAS
CSCD
1999年第2期20-24,共5页
Industrial Microbiology
关键词
聚Β-羟基丁酸
动胶菌
优化培养基
发酵条件
poly β hydroxybutyric acid (PHB)
Zoogloea
op timized optimal culture medium
fermentation conditions
