摘要
茶多糖具有多种重要的生物活性。本研究采用响应面法优化茶籽多糖的超临界CO2提取工艺,运用小星点设计考察萃取时间(30～150min)、压力(15～45MPa)、温度(40～80℃)及夹带剂乙醇浓度(50%～90%)四因素对茶籽多糖得率的影响。响应面分析表明,茶籽多糖得率与四因素关系符合二次模型。时间和夹带剂乙醇浓度的一次项和二次项、压力的二次项以及时间和压力的交互作用对茶籽多糖得率具有显著影响。该二次多项式模型用后退法进行逐步回归得到简化模型,依据简化后的模型在各因素设定范围内获得最佳提取工艺为:时间150min,压力45MPa,温度60℃,夹带剂乙醇浓度为65%。该条件下进行三次重复试验,茶籽多糖的实际平均得率为13.23%,与预测值13.96%无显著差异。
Tea polysaccharide has various important biological activities.The supercritical CO2 extraction process with the ethanol aqueous solution as solvent modifier was used to extract the polysaccharide from tea seed,and the response surface methodology（RSM） was used to optimize the process parameters.Four process parameters,extraction time（30～150 min）,pressure（15～45 MPa）,temperature（40～80℃） and the ethanol content in modifier（50%～90%） were selected as four independent variables.A small central composite design（small CCD） was used to study the effects of the above four independent variables on the yield of the extracted tea seed polysaccharide.The quadratic formula was obtained by response surface analysis between the yield of the extracted tea seed polysaccharide and the four parameters.The linear and quadratic term of time and ethanol concentration,the quadratic term of pressure and the interaction between time and pressure have significant effects on the yield of tea seed polysaccharide.The second-order polynomial model was simplified by using a backward stepwise method,and then the optimum parameters within the experimental ranges were determined to be at 150 mim,45 MPa,60℃ and with 65% ethanol aqueous solution.Under these conditions,the average value of the actual yield of the tea seed polysaccharide in three replicated experiments is 13.23%,and this value is not significantly different from the value of 13.96% predicted by the model.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2010年第5期897-902,共6页
Journal of Chemical Engineering of Chinese Universities
关键词
超临界CO2
茶籽
多糖
响应面分析
小星点设计
supercritical carbon dioxide
tea seed
polysaccharide
response surface methodology（RSM）
small central composite design（small CCD）
