摘要
目的观察血清标本在相同温度下保存不同时间时检测得9项生化指标的变化,并分析造成误差的原因。方法对在-18～-22℃冰箱保存1周、2周、1个月的血清标本分别进行丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、总蛋白(TP)、清蛋白(ALB)、葡萄糖(GLU)、肌酐(CR)、尿酸(UA)、尿素氮(BUN)测定,并将各时间点的测定值与即刻测定值比较。结果血清标本保存1周起ALT、TBIL、BUN检测值开始下降,除AST、ALB、GLU、CR外,其余指标的检测值与即刻检测值比较差异有统计学意义(P<0.05);血标本放置2周时,除UA外其余指标的检测值与即刻检测值比较差异有统计学意义(P<0.05);血标本放置1个月时,除TBIL外其余指标的检测值与即刻检测值比较差异有统计学意义(P<0.05)。冰冻1周的血标本检测的ALT、TBIL、BUN值与即刻的检测值比较,已超出实验室允许的误差范围;而冰冻2周的血标本检测的AST、TP、ALB、GLU、CR、UA值仍在实验室允许的误差范围之内。血标本冷冻1个月时除AST、TBIL外,其余指标的检测结果均超出实验室允许的误差范围。结论经-18～-22℃相同温度冰箱中冷冻保存的血清标本其生化检测值会发生改变,测定酶类和BUN的血清标本不适宜冰冻保存,而TBIL标本应避光保存。
Objective 9 items of biochemical tests were measured after serum samples was stored in same condition for different time.Observe the indicator changes and analyze its causes.Methods Serum samples were each measured on ALT,AST,TBIL,TP,ALB,GLU,CR,UA and BUN biochemical items after storing in-18~22℃ refrigerator for 1w,2w and 1m time.Compare the testing results of each time with the result of ST.Results The ALT,TBIL and BUN tests values dropped after 1w store in-18^-22 ℃ refrigerator,and difference between the testing results and immediate testing results of indicators （except AST,ALB,GLU and CR） was significant（P0.05）.After 2w,difference between the testing results and immediate testing results of indicators （except UA） is significant（P0.05）.After 1m,difference between the testing results and immediate testing results of indicators （except TBIL） was significant（P0.05）.The testing results of ALT,TBIL and BUN were out of the acceptable range compared with the immediate testing results after 1w in refrigerator.The testing results of AAST,TP,ALB,GLU,CR and UA were within the acceptable range after 2w in refrigerator.Testing results of all indicators except AST and TBIL are all out of acceptable range after 1m in refrigerator.Conclusion 9 items biochemical values of serum samples were changed in 1w,2w and 1m of-18~22 ℃ keeping.The enzymes or BUN sample is not appropriate in frozen store,while the TBIL serum samples should be kept without light.
出处
《中国全科医学》
CAS
CSCD
北大核心
2010年第30期3427-3428,共2页
Chinese General Practice
关键词
生化标本
保存
检验误差
Biochemical sample
Store
Laboratory error
