摘要
为了利用家蚕丝素蛋白轻链基因(Fib-L)及启动子元件开展转基因研究,克隆了家蚕品种797的Fib-L cDNA及启动子,并比较了6种不同品种来源的Fib-L基因的cDNA序列。结果表明,轻链基因cDNA长为786 nt,编码了包括由18个氨基酸组成的信号肽在内的共262个氨基酸的蛋白质前体;启动子序列分析显示其包括典型的TATA盒和类CAAT盒序列以及丝腺特异性结合位点等。用Fib-L启动子控制报告基因Egfp,进行家蚕BmN细胞内的瞬时表达研究,结果表明,Fib-L启动子驱动的Egfp报告基因可以在BmN细胞瞬时表达。
To conduct the transgenic research on the basis of fibroin light chain（Fib-L） and its promoter,Fib-L cDNA and its promoter of silkworm 797 were cloned and analyzed with that of other five silkworm strains.The results showed that the length of cDNA was 786 nt,which encoded 262 amino acids precursor protein including 18 amino acid signal peptides,meanwhile,the promoter contained the typical TATA box,CAAT like box and silk gland special binding site,and Egfp driven by Fib-L promoter can be transient expression in BmN cell through transfection.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2010年第10期68-72,共5页
Journal of Northeast Agricultural University
基金
国家高技术研究发展计划"863计划"项目(2006AA10A119)
江苏科技大学博士基金项目(35180905)
