摘要
目的：探讨β揽香烯对小鼠Ｂ１６黑色素瘤细胞粘附和运动能力的影响。方法：采用细胞培养、电镜及划痕染料示踪技术，研究了β榄香烯对小鼠Ｂ１６黑色素瘤细胞粘附与运动能力、间隙连接结构及其间隙连接通讯功能的影响。结果：２０～４０μｇ／ｍｌ的β榄香烯可显著抑制Ｂ１６细胞对纤维结合蛋白和层粘连蛋白两种基质的粘附，抑制Ｂ１６细胞的运动，延迟吸管尖部所致细胞单层损伤的愈合。结论：提示β榄香烯对Ｂ１６细胞粘附和运动能力的抑制可能是其抗肿瘤转移作用的机制之一。
The influences of β elemene on the abilities of adhesion and motility and on the gap junctional intercellular communication(GJIC) of mice melanoma B 16 cells were studied in vitro.It was found that β elemene in 20～40 μg/ml inhibited significantly the adhesion of B 16 cells to fibronectin and lamin,and that the drug in 10～40 μg/ml suppressed the movement of B 16 cells in vitro and delayed healing of cell monolayer damage.No obvious effect of β elemene in above concentration on gap junction structure and GJIC of B 16 cell was observed.It is suggested that the suppression of β elemene on the adhesion and motility of B 16 cells may be an important mechanism of the drug inhibiting tumor metastasis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1999年第3期195-197,共3页
Cancer Research on Prevention and Treatment
关键词
Β-榄香烯
黑色素瘤细胞
粘附
运动
β elemene
B
16 melanoma cells
adhesion
motility
gap junctional intracellular communication
