摘要
研究了在氧化镍纳米粒子改性石墨电极(GE)上肌红蛋白(Mb)的直接电化学行为,并制备了新型H2O2传感器。在0.1mol/L磷酸盐缓冲溶液(PBS,pH7.0)中,肌红蛋白有稳定而明确的氧化还原峰,电子转移速率常数为6.48/s;式量电位为-0.34V(vs·SCE),表面覆盖量8.06×10-10mol/cm2。二甲亚砜(DMSO)的存在对加速肌红蛋白分子与电极之间的电子传递起了重要作用。光谱分析表明:固定在Mb/NiO/DMSO膜中肌红蛋白能保持其生物活性,对H2O2有电催化活性,电催化响应与H2O2浓度呈线性关系,线性范围为0.8～24μmol/L;检出限为0.039μmol/L。对H2O2的表观米氏常数为0.21mmol/L,灵敏度为417mA cm2L/mol,呈现出高亲和性。
Direct electrochemistry of myoglobin （ Mb） immobilized on a nanometer-sized NiO nanoparticles matrix modified graphite electrode （ GE） and preparation of novel hydrogen peroxide biosensor were studied. The immobilized Mb displayed a couple of stable and well-defined redox peaks with an electron transfer rate constant of 6. 48 s -1 and a formal potential of - 0. 340 V（ vs·SCE） in 0. 1 mol/L PBS （ pH 7. 0） . The total surface concentration was 8. 06 × 10 -10mol/cm2. Dimethyl sulfoxide （ DMSO） could play an important role in the electron transfer between Mb and the electrode. Spectroscopy analysis of the Mb/NiO/DMSO film showed that the immobilized Mb could retain its natural structure. The electrocatalytic response showed a linear dependence on the H2O2 concentration ranging from 0. 8 to 24 μmol/L with a detection limit of 0. 039 μmol/L （ 3σ） . The apparent Michaelis Menten constant KMapp for H2O2 sensor was estimated to be 0. 21 mmol/L and the sensitivity was 417 mA cm2 L/mol,which showed a high affinity.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2010年第11期1533-1537,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20905010)
南京大学“生命分析化学教育部重点实验室”2008年度开放课题项目(No.KLACLS08007)
常熟市科技局项目(No.CS200808)
常熟理工学院毕业设计(论文)团队课题项目资助
