摘要
目的：探讨皮肤创面两种神经肽（ＣＧＲＰ，ＳＰ）的起源及在创面愈合中的作用．方法：运用免疫组化观察大鼠烫伤皮肤创面上述神经肽的分布，运用原位杂交技术观察它们的ｍＲＮＡ表达情况，运用体外细胞培养技术研究其对成纤维细胞增殖影响．结果：皮肤烫伤后早期一些炎性细胞从创面和创周皮肤血管游出，并对ＣＧＲＰ和ＳＰｍＡｂ免疫反应呈阳性．伤后１２ｈ该细胞与皮肤内的神经纤维关系密切，伤后２４ｈ细胞破碎并释放出相应的神经肽免疫反应阳性的颗粒性物质．原位杂交方法显示：烫伤后６ｈ该部位相同大小细胞内表达神经肽（ＳＰ，ＣＧＲＰ）的ｍＲＮＡ．进一步实验证实ＣＧＲＰ和ＳＰ对体外培养成纤维细胞有刺激增殖作用，且相互间具有协同作用．结论：烫伤皮肤创面神经肽ＣＧＲＰ和ＳＰ可能由来自血液的炎性细胞合成释放。
AIM: To investigate the origin of neuropeptides (SP, CGRP) and their effects on wound healing. METHODS: By the use of immunocytochemical technique, the distribution of CGRP and SP immunoreactive cells was observed in and around the early phase cutaneous wounds. Using in situ hybridization technique, the expression of neuropeptides (SP, CGRP) mRNA in cells was studied. The effects of the CGRP and SP on the proliferation of 3T3 cells in vitro were investigated. RESULTS: The immunoreactive cells migrated from blood vessels, were associated with CGRP and SP containing nerve fibers in the early phase post burn, and released CGRP and SP immunoreactive materials into the dermis 24 hrs post burn. Using in situ hybridization histochemistry, the CGRP mRNA and preprotachykinin A (PPTA) mRNA encoding SP were expressed in inflammatory cells around wounds 6 hrs post burn. Further experiment demonstrated that CGRP and SP as mitogen alone or together stimulated DNA synthesis of cultured 3T3 cells in vitro . CONCLUSION: It is suggested that CGRP and SP may be synthesized and released by inflammatory cells from blood vessels and may play an important role in cutaneous wound healing.
出处
《第四军医大学学报》
1999年第5期409-411,共3页
Journal of the Fourth Military Medical University
基金
全军九五重点课题
关键词
烫伤
皮肤创面
炎性细胞合成
CGRP
SP
calcitonin gene related peptide
Substance P
histocytochemistry
in situ hybridization
