摘要
目的研究Ｌ精氨酸（Ｌａｒｇ）对５／６肾大部切除大鼠（ＳＮｘ）肾小管、间质αＳＭＡ表达的影响及其与细胞外基质合成、肾功能的关系。方法实验分４组：ｓｈａｍ；ｓｈａｍ＋Ｌａｒｇ；ＳＮｘ；ＳＮｘ＋Ｌａｒｇ。大鼠于术后即由饮水中给予１０ｇ·Ｌ－１Ｌａｒｇ。１ｍｏｎ后，大鼠处死，肾组织经免疫组化半定量分析肾小管间质αＳＭＡ以及ｃｏｌａｇｅｎⅢ、ｃｏｌａｇｅｎⅣ、ｆｉｂｒｏｎｅｃｔｉｎ的表达程度。用ＭａｓｏｎＴｒｉｃｈｒｏｍｅ染色切片来判断肾小管、间质损害程度。结果Ｌａｒｇ明显增加ＳＮｘ大鼠肾小管、间质αＳＭＡ及ｃｏｌａｇｅｎⅢ、ｃｏｌａｇｅｎⅣ、ｆｉｂｒｏｎｅｃｔｉｎ表达，αＳＡＭ表达程度与上述细胞外基质成分之间呈显著的正相关（Ｐ分别＜００１）。更为重要的是αＳＭＡ表达程度尚与肌酐清除率呈负相关（Ｐ＜００１），而与小管、间质损害程度呈正相关（Ｐ＜００１）。结论Ｌａｒｇ可能激活ＳＮｘ大鼠肾小管、间质成肌纤维细胞（ＭＦＢ），并促进肾脏纤维化。
AIM To investigate
the possible role of Larginine(LA) on the tubulointerstitial expression of smooth muscle actin(
SMA) and its relationship with the interstitial expression of extracellular matrix components
(collagen ,collagen , fibronectin) in rats with 5/6 subtotal nephrectomy(SNx). METHODS The
experiment includes four groups:sham; sham+Larg; SNx; SNx+Larg. Rats were treated by 10
gL-1 Larginine for one month after they were subjected to the operation. At the end of
experiment, the kidneys were processed for immunohistochemical detection of SMA, collagen
collagen and fibronectin, as well as Masson Trichrome staining. RESULTS Larg significantly
increased the tubulointerstitial expression of SMA in rats with SNx(P<005). Meanwhile, Lare
significantly increased the tubulointerstitial staining for collagen , collagen , and
fibronectin(P<005 respectively). Furthermore, the extent of expression of SMA positively
correlated with the expression of the above extracellular matrix components(P<001
respectively). More importantly, the expression of Larg correlated well with the decline of Ccr
and tubulointerstitial lesions (P<001 respectively). CONCLUSION This experiment suggested
that Larg might activate the tubulointerstitial myofibroblast which then contribute to the
development of renal fibrosis in SNx rats.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1999年第2期127-131,共5页
Chinese Pharmacological Bulletin
