摘要
目的通过检测直肠腺癌中NDRG1蛋白的改变,探讨其在直肠腺癌演进中的作用。方法应用免疫组织化学法检测80例直肠腺癌和12例直肠腺瘤组织中NDRG1和E-cadherin蛋白的表达情况,应用免疫印迹技术(Western blot)检测NDRG1基因编码蛋白在上述组织中表达水平的变化。结果 (1)免疫组织化学结果:NDRG1和E-cadherin蛋白在直肠腺癌组织中的阳性率分别为77.5%和53.8%,而直肠腺瘤组织中阳性率分别为50.0%和91.7%,差异均有统计学意义(P<0.05)。NDRG1蛋白表达在TNMⅢ～Ⅳ期组和淋巴结转移组阳性率均为95.0%,明显高于TNMⅠ～Ⅱ期组和无淋巴结转移组(60.0%);在浆膜浸润组为82.8%,高于肌层以内浸润组(63.6%),差异有统计学意义(P<0.05)。E-cadherin蛋白阳性率在高-中分化直肠腺癌组为58.8%,明显高于低分化组(25.0%);而在TNMⅢ～Ⅳ期组和淋巴结转移组(32.5%)低于TNMⅠ～Ⅱ期和无淋巴结转移组(75.0%),差异有统计学意义(P<0.05)。(2)Western blot定量检测NDRG1基因编码蛋白结果与免疫组织化学结果基本一致。结论 NDRG1蛋白可能作为潜在癌基因在直肠腺癌进展过程中起一定作用,联合检测NDRG1和E-cadherin蛋白的表达有助于判断直肠腺癌生物学行为。
Objective To investigate the role of NDRG1 protein in rectal adenocarcinoma development.Methods The expressions of NDRG1 and E-cadherin proteins were detected by immunohisto-chemical technique and Western blot,in 80 cases of rectal adenocarcinoma and 12 cases of rectal adenoma tissues.Results（1）The positive rates of NDRG1 and E-cadherin proteins were significantly diferent between the groups of adenoma（50.0% and 91.7%）and adenocarcinoma（77.5%and 53.8%） respectively（P0.05）.Positive rate of NDRG1 protein（95.0%） was higher in the groups of TNMⅢ～Ⅳ and lymph-node metastasis than that in the groups of TNMⅠ～Ⅱand lymph-node metastasis-free（60.0%）（P0.05）.Expression of NDRG1 protein was more increased in group of serosa invasion（82.8%） than that in group of invasion within the muscular layer（63.6%）（P0.05）.Positive rate of E-cadherin（58.5%） was higher in the group of well-moderately differentiated rectal adenocarcinoma than that in the group of the poorly differentiated rectal adenocarcinoma（25.0%）.Meantime positive rate was lower in the groups of lymph-node metastasis and TNMⅢ～Ⅳ（32.5%）than that in the groups of without lymph node metastasis and TNMⅠ～Ⅱ（75.0%）（P0.05）.（2）The expression levels of NDRG1 protein detected by western blot were consistent with the results by IHC.Conclusion It was suggested that NDRG1 might be contribute to the progression of rectal adenocarcinoma as a potential oncogene.The identification of both NDRG1 and E-cadherin proteins might be advantageous to analyse biological behaviors of rectal adenocarcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第10期1166-1169,1178,共5页
Cancer Research on Prevention and Treatment
基金
辽宁省自然科学基金资助项目(002073)
