摘要
目的观察CO2对宫颈癌HeLa细胞系抗失巢凋亡能力的影响。方法以宫颈癌HeLa细胞系为研究对象,实验组给予CO2持续通气4小时,压力为8mmHg,对照组HeLa细胞不进行CO2通气,两组细胞同时设贴壁培养组和悬浮培养组进行培养24h、72h、120h。利用软琼脂集落形成实验、PolyHEMA悬浮培养、流式细胞技术、观察体外培养过程中CO2对宫颈癌HeLa细胞系增殖和抗失巢凋亡的影响。结果实验组与对照组均具有凋亡能力和抗失巢凋亡能力,实验组的增殖能力和集落形成明显优于对照组(P<0.01),但实验组抗失巢凋亡能力明显低于对照组。结论 CO2能够促进宫颈癌HeLa细胞集落形成,具有较强的失巢凋亡能力,但不能增强抗失巢凋亡能力。
Objective To observe the effect of CO2 on the anti-anoikis of HeLa cell（cervical cancer）.Methods HeLa cells were randomly divided into experimental group and control group.In experimental group,CO2 was insufflated into the containers by pressures of 8 mm Hg for 4 hours.Control group was not treated by CO2.Two groups of cells were cultured for 24h,72h and 120h in adherent and suspension methods.The effect of CO2 on the proliferation and anti-anoikis of HeLa cells were detected through soft agar colony formation experiments,Poly HEMA suspension culture and Flow cytometry assay.Results The abilities of apoptosis and anti-anoikis were observed in 2 groups.The proliferation and colony formation capabilities were stronger in experimental group than that in control group,but the anti-anoikis of experiment group was significantly lower than that of the control group（P0.01）.Conclusion CO2 was able to improve the colony formation and anoikis of HeLa cells,no a great impact,however,on anti-anoikis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第10期1132-1135,共4页
Cancer Research on Prevention and Treatment
基金
广西壮族自治区科学技术自然基金资助项目(桂科基0575088)
关键词
宫颈癌
HELA细胞
失巢凋亡
增殖
浸润
转移
Cervical cancer
HeLa cells
Anoikis
Proliferation
Invasion
Metastasis
