摘要
目的 研究血管生成素-1（angiopoietin-1,Ang-1）及其受体Tie-2在多发性骨髓瘤（MM）患者和骨髓瘤细胞株RPMI8226的表达情况,探讨其意义.方法 采用RT-PCR和Western blot方法检测112例MM患者、24例非肿瘤患者（对照组）以及RPMI8226细胞株中Ang-1及其受体Tie-2的表达水平,分析阳性率及表达水平在MM患者与对照组、MM不同分期间的差异,探讨Ang-1表达与MM患者骨髓微血管密度、临床分期及预后的关系.结果 MM组Ang-1表达阳性率、表达水平均显著高于对照组（P＜0.05）;Ang-1表达阳性率在初治组和复发/难治MM组的差异无统计学意义（P＞0.05）,但复发/难治MM组的Ang-1表达水平高于初治组（P＜0.05）;Tie-2 mRNA仅在12例MM患者中检出,对照组未检出.MM患者骨髓微血管密度（25.21±0.80）明显高于对照组（5.23±0.20）（P＜0.01）;Ang-1阳性患者的骨髓微血管密度（32.98±1.70）高于Ang-1阴性患者（16.55±1.30）（P＜0.05）;Ang-1表达阳性率在Ⅱ期和Ⅲ期MM患者中差异无统计学意义（52.1%对60.9%,P＞0.05）,但Ⅲ期MM患者Ang-1蛋白的表达水平（0.40±0.07）明显高于Ⅱ期患者（0.22±0.04）（P＜0.05）;初治患者中,治疗无效组Ang-1表达阳性率（70.0%）明显高于有效组（19.1%）（P＜0.01）.结论 Ang-1在MM患者中高表达;Ang-1表达与MM临床分期、预后判断及靶向治疗有关.
Objective To study the expression of angiopoietin-1（Ang-1）and its receptor Tie-2 in multiple myeloma（MM）patients and RPMI8226 cells,and analyze the significance of Ang-1 expression and its relevance to the tumorigenes and development of MM.Methods RT-PCR and Western blot were used to detect the expression of Ang-1 and Tie-2 in bone marrow（BM）samples from 112 MM patients and 24 control subjects,and in RPMI8226 cells.The expression levels of Ang-1 in different groups and disease stages were analyzed.Results The positive rate and expression level of Ang-1 were significantly higher in MM group than in control group（P 〈0.05）.The positive rates of Ang-1 were not significantly different between newly diagnosed and relapsed/refractory MM groups,but its expression level was significantly higher in the latter group than in the former group（P 〈0.05）.Tie-2 was detected only in 12 MM patients and did not in control group and RPMI8226 cells.Microvessel density in BM samples were significantly higher in MM group than in control group（25.21±0.80 vs 5.23±0.20,P〈0.01）,and were higher in Ang-1-positive MM group than in Ang-1-negative M M group（32.98 ± 1.70 vs 16.55 ± 1.30,P 〈 0.05）.The positive rates of Ang-1 protein were not significantly different between stage Ⅱ and stage Ⅲ MM（52.1% vs 60.9%,P〉0.05）,but the expression level of Ang-1 protein was higher in stage Ⅲ than that in stage Ⅱ MM（0.40 ±0.07 vs 0.22 ±0.04,P 〈0.05）.In the newly diagnosed MM patients,the positive rate of Ang-1 protein in PD patients was significantly higher than in PR and MR patients（70.0% vs 19.1%,P 〈0.01）.Conclusion High expression of Ang-1 is found in MM patients and RPMI8226 cells,and its expression is associated with the disease stage,prognosis and targeted therapy of MM.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2010年第10期654-658,共5页
Chinese Journal of Hematology
基金
河北省自然科学基金（C2008001097）
