摘要
目的：通过不同浓度脑源性神经营养因子（BDNF）对神经干细胞（NSCs）增殖与分化的影响,探讨合适的诱导时间和诱导浓度.方法：按照BDNF的浓度分为对照组,实验组2、20和200ng/ml BDNF.MTT法检测不同浓度BDNF在诱导后不同的时间点（1、3、5、7d）对细胞增殖的影响,免疫细胞化学法检测神经元的标记物-神经元特异性烯醇化酶（NSE）,并计算各组在不同时间点的神经元分化率.结果：不同浓度的BDNF实验组在诱导后不同时间（1、3、5、7d）MTT法所测OD值与对照组相比差异均无统计学意义.随着BDNF浓度的增加,神经干细胞分化为神经元的比率呈增高的趋势,差异有统计学意义.在分化的时间上,各组均在第3天时达到最高,其中实验组最高接近80%,而对照组仅为35%.结论：BDNF对NSCs的增殖无明显作用,BDNF能促进NSCs向神经元方向分化,其分化的比率在一定范围内呈剂量依赖性.
Objective： To investigate the effects of brain-derived neurotrophic factor （BDNF） in different concentrations on the proliferation and differentiation of neural stem cells （NSCs） and to seek the optimal concentration and time of BDNF in the differentiation of NSCs. Methods： The cultured NSCs were divided into four groups including 0 ng/ml group （control group） and 2,20,200 ng/ml groups （experimental groups） according to the concentration of BDNF added into culture medi- um. MTT method was used to investigate the effect of BDNF in different concentrations on the proliferation of NSCs at different time point after induction. The expression of neuron specific enolase （NSE）, a specific marker of neuron, was detected by immunocytochemistry and the differentiation rate of NSE-positive neuron was calculated. Results： There were no significant differences in OD values detected by MTT assay between the control group and the experimental groups at each time point after induction. With the increasing concentration of BDNF, the percentage of NSE positive cells also increased and the differences were statistically significant. The proportion of NSE-positive neurons in each group reached its peak on the 3rd day after induction, which was approximately 80%, but only about 35% in the control group. Conclusion： BDNF has no apparent effect on the proliferation of NSCs. BDNF noticeably contributes to the differentiation of NSCs to neuron in a concen- tration-dependent manner.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2010年第5期648-651,共4页
Chinese Journal of Anatomy
基金
河北省教育厅课题（2005116）
河北省科技厅课题（072761895）
关键词
脑源性神经营养因子
神经干细胞
增殖
分化
brain-derived neurotrophic factor
neural stem cells
proliferation
differentiation
