摘要
目的探讨动态观测ＨＣＶ感染指标及序列变异的意义。方法ＥＬＩＳＡ方法检测抗ＨＣＶＩｇＧ、ＩｇＭ，ＲＴＰＣＲ方法检测ＨＣＶＲＮＡ，ＰＣＲ产物直接测序。结果６例感染ＨＣＶ的血透析患者抗ＨＣＶＩｇＧ的表现类型分为持续阴性及持续阳性两种；抗ＨＣＶＩｇＭ有四种表现：一过性阳性、间隙性阳性、持续阴性及持续阳性；ＨＣＶＲＮＡ检测多为间断阳性；同一患者不同时期感染的ＨＣＶ株Ｅ２／ＮＳ１区序列间存在差别，且ＨＶＲ１区基因变异与有无抗体生成有关。结论抗ＨＣＶＩｇＧ、ＩｇＭ、ＨＣＶＲＮＡ的动态检测对说明体内有无病毒存在，疾病有无慢性化倾向有重要的意义。ＨＣＶＥ２／ＮＳ１区基因变异对提示病毒逃避机体免疫清除，疾病向慢性化方向发展有指导意义。
Objective To study the clinical significance of HCV infectious index and sequence variation. Methods Anti HCV IgG、 IgM and HCV RNA were detected by ELISA and RT PCR, respectively. Results Anti HCV IgG detected in Six dialysis patients infected by HCV could be divided into persistent positive type and persistent negative type, whereas four types of anti HCV IgM were observed, which were etransient positive; intermittent positive, persistent positive and persistent negative HCV RNA were found intermittent positive in most cases. The sequence of HCV E2/NS1 region varied in different period at the same patient, and it seemed that there was certain relationship between HVR1 divergence and antibody geveration.Conclusion Dynamic study of anti HCV IgG,IgM and HCV RNA have important chinical significance in determining whether there exists virus in the body and chronicity tendency of disease in patients. The variation of HCV E2/NS1 gene which confers escape from immunosurveillance of the host may be the reason making the disease chronicity.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第2期100-103,共4页
Chinese Journal of Infectious Diseases
