摘要
采用组织块分离法从陕北野生甘草根中分离出21株内生菌,其中细菌12株,真菌6株,放线菌3株。为明确内生菌代谢产物的抑菌活性,采用抑制菌丝生长速率法和组织测定法系统测定了其发酵液对植物病原真菌的抑制作用。结果表明,在离体条件下,甘草内生菌发酵液对供试的7种植物病原菌菌丝生长均有一定的抑制作用,其中Z4和F1菌株的发酵液对供试病原菌菌丝生长的抑制作用较强,特别是对苹果干腐病菌和烟草赤星病菌的抑制作用最强,抑制率分别可达92.36%、90.16%和91.20%、90.43%,EC50分别仅为54.38、90.31和57.50、89.58 mg.L-1;组织法测定中,发酵液稀释10倍后,Z4和F1菌株对苹果干腐病的保护作用分别为75.39%和73.13%,治疗效果分别为67.61%和60.75%。
21 strains of endophytes were isolated from the roots of wild liquorice in North Shaanxi by the method of tissue separation.They included 12 strains of endophytic bacteria,6 strains of endophytic fungi and 3 strains of endophytic actinomycetes.The antifungal activity of the supernatant fermentation from all of endophytes were tested in vitro.The results indicated that the supernatant fermentation of all inhibited hyphae growth of 7 species investigated plant-pathogenic fungal in vitro.Especially that of Z4 strain and F1 strain could markedly inhibite the hyphae growth to Botryosphaeria berengeriana and Alternaria alternata.Their inhibition rate against Botryosphaeria berengeriana and Alternaria alternate were 92.36%,90.16% and 91.20%,90.43%,respectively.The EC50 value against Botryosphaeria berengeriana and Alternaria alternata were only 54.38,90.31 and 57.50,89.58 mg·L-1,respectively.In addition,the protective effect of the fermentation products diluted 10 times against Botryosphaeria berengeriana were 75.39% and 73.13% on apple and the therapeutic effect were 67.61% and 60.75%,respectively.It was showed that the further investigating of antifungal activity in endophytes Z4 and F1 were necessary.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第10期2110-2115,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省教育厅专项科研计划项目(08JK494)
延安市科学技术研究发展计划项目(2008ks-21)
延安大学科研基金资助项目(YD200653)
延安大学大学生科技创新训练计划项目(D2007-40)
关键词
甘草
内生菌
抑菌活性
liquorice
endophyte
antifungal activity
