摘要
以筛选的6对IRAP引物对15个元帅无性系芽变和16个富士无性系芽变进行了鉴定,以揭示苹果无性系变异的机理。结果显示,所有引物在两种无性系芽变中均扩增出丰富的条带,表现出多态性。SAHN聚类分析结果显示,以相似系数0.65为阈值,元帅芽变无性系和富士芽变无性系各自聚为一类;以相似系数0.83为阈值,供试的15个元帅无性系变异可聚为4类:第一类包括‘元帅’、‘居家店短红星’、‘艳红’、‘米勒矮生’、‘矮壮’、‘红星’、‘红冠’、‘矮威尔’、‘早红星’和‘华帅一号’,第二类包括‘玫瑰红’、‘奥勒冈矮生’和‘矮红’;‘新红星’和‘哈蒂.勃莱特’分别独自成类;以相似系数0.89为阈值,供试的16个富士芽变无性系可聚为4类,第一类包括‘长富3’、‘盛放富1’、‘长富6’、‘群富1’、‘烟富1’、‘竽井Ⅱ系’、‘岩手Ⅰ系’、‘富士Ⅰ系’、‘红王将’、‘长富7’和‘长野Ⅰ系’,第二类包括‘富士’和‘秋富5’,第三类包括‘斋藤Ⅱ系’和‘秋富2’,‘秋富1’独自成类。研究表明,逆转座子在苹果基因组内的插入方向具有随意性,转座插入和逆转座子间重组是引起苹果无性系变异的两个重要机制,本试验所开发的引物是进行苹果无性系变异研究的有效工具。
Fifteen Red Delicious bud sport clones and sixteen Fuji bud sport clones were distinguished by 6 IRAP molecular markers based on primers designed with Ty1-copia-like retrotransposons LTRs.The polymorphic,clear bands amplified,could identify the Red Delicious bud sport clones and Fuji bud sport clones.15 Red Delicious bud sport clones and 16 Fuji bud sport clones were clustered together,respectively,based on SAHN analysis using similarity 0.65 as the standard.The phylogenetic relationship among 15 Red Delicious bud sport clones based on SAHN analysis showed that Red Delicious bud sport clones could be grouped into 4 families using similarity 0.83 as the standard.The first group was composed of 10 bud sport clones,which were Red Delicious,Jujiadian spur starking,Starspur Ultra Red Delicious,Miller Spur Delicious,Hardispur Delicious,Starking,Richard,Well spur Delicious,Early Red Delicious and Chinese Marshal 1.The second group comprised Meiguihong,Oregon spur Delicious and Show Red.Starkrimson and Hardi Bolaite were as a single group,respectively.Similar to Red Delicious bud sport clones,16 Fuji sport clones could be grouped into 4 families based on similarity 0.89.The first group contained 11 sport clones,which were Nagafu 3,Shengfangfu 1,Nagafu 6,Qunfu 1,Yanfu 1,Yujing Ⅱ,Yanshou Ⅰ,Fuji Ⅰ,Hongwangjiang,Nagafu 7 and Changye Ⅰ.The second group comprised Fuji and Akifu 5.The third included Zhaiteng Ⅱ and Akifu 2.Akifu 1 was as a single group.The results showed that the insertion orientations of retrotransposons were independent in apple genome.Transpositional insertion and recombination between retrotransposons were two of important mechanisms inducing apple somatic cell variation.The primers developed in the experiment provided useful tools for identification of apple bud sport clones,which laid the foundations for the further study of the mechanisms of apple bud mutations.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第10期1952-1958,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(30740034
31071776)
安徽农业大学稳定与引进人才科研启动专项基金项目(wd2006-8)
关键词
苹果
IRAP标记
芽变
apple
IRAP molecular markers
bud mutations
