摘要
采用聚合酶链反应（ＰＣＲ）方法改造鲑鱼（Ｏｎｃｏｒｈｙｎｃｈｕｓｔｓｃｈａｗｙｔｓｃｈａ）生长激素ｃＤＮＡ。改造后的ｃＤＮＡ去除了编码生长激素成熟肽ｃＤＮＡ以外的信号肽序列及５′端和３′端的非翻译序列，加入一起始密码，并在改造后的基因两端引入ＢａｍＨＩ识别序列各１个。亚克隆后对改造过的基因进行了序列分析。
A polymerase chain reaction (PCR) method was used to modify the growth hormone cDNA of Oncorhynchus tschawytscha.The modified cDNA scquence contains an initiator codon and a completely coded mature peptide sequence of sGH for chinook trout,and deletes putative signal sequence and 5′ & 3 ′ untranstated regions.For easy to subclone,one Bam H Ⅰ site was added to each side of reformed gene.After cDNA subcloned,the modified gene sequcnce was analyzed.
出处
《中国水产科学》
CAS
CSCD
1999年第1期41-44,共4页
Journal of Fishery Sciences of China
