摘要
以４甲基伞形酮酰βＤ葡糖醛酸苷（４ｍｅｔｈｙｌｕｍｂｅｌｉｆｅｒｙｌβＤｇｌｕｃｕｒｏｎｉｄｅ，４ＭＵＧ）为底物，将其结合于选择性固体培养基中，采用荧光法检测细菌是否产生特异性的β葡糖苷酸酶作为鉴别细菌的依据。结果表明：大肠杆菌在脱氧胆酸钠培养基（ＤＯＣ培养基）中经３７℃培养９～１１ｈ，其菌落在３６５ｎｍ波长紫外光激发下可产生明显的荧光，而其它几种常见的革兰氏阴性肠杆菌（志贺氏菌、克雷伯氏菌、沙门氏菌、柠檬杆菌和弧菌）和铜绿假单胞菌虽能在其中生长，但不产生荧光；在麦康凯培养基中，大肠杆菌菌落出现荧光的时间明显延迟，其它革兰氏阴性菌也仍然不产生荧光。上述两种培养基对耶尔森氏菌和金黄色葡萄球菌的生长具有抑制作用。本试验结果提示，ＤＯＣ培养基可试用于大肠杆菌的鉴定，具有快速、简便和特异性强的特点。
methyl umbelliferyl β D glucuronide (4 MUG) was incorporated into the deoxycholate sodium agar medium (DOC agar) to detect the production of β glucuronidase by the bacteria using the fluorogenic assay.All the Escherichia coli strains tested could grow well in DOC agar after incubation at 37℃ for 9～11 hours and produce fluorescence upon stimulation of their colonies by the 365 nm ultravionlet spectrum while the other gram negative enterobacters (Shigella,Klebsiella,Salmonella,Citrobacter and Vibrio ) and Pseudomonas aerugionsa strains could grow on the medium with no fluorescenece.On the MacConkey agar incorporated wiht 4 MUG, E. coli grew well but with delayed fluorescence, while the other gram negative bacteria also did not exhibit fluorescence irrespective of their growth.Both media were inhibitory to the growth of Staphylococcus aureus and Yersinia .The preliminary study suggests that DOC agar medium may be used with simplicity for rapid and specific detection of E.coli.
出处
《浙江农业学报》
CSCD
1999年第2期92-95,共4页
Acta Agriculturae Zhejiangensis
基金
IFS资助项目
关键词
荧光检测法
β-葡糖苷酸酶
牛
乳房炎
大肠杆菌
fluorogenic assay
4 methyl umbelliferyl β D glucuronide
β glucuronidase
Eseherichia coli
