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基于矿石纳米材料的DNA转化的机理初探及方法改进 被引量：2

Mechanism of DNA transformation based on mineral nanofibers and method improvement
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摘要 一种价格便宜、资源丰富和对人体无害的矿石纳米材料——海泡石,用于微生物DNA转化。该法简便、快速、对人体健康无害,但对这种转化方法机制的理解还有很多问题。通过小片段RNA竞争试验,发现了与先前报道不同的转化机制。同时,对该法进行了优化,结果可以实现对冷藏1个月的大肠杆菌EscherichiacoliDH5α单菌落直接转化,无需感受态制备和处理后的温育过程,可得到比钙转高的转化率。由于可优化的参数很多,所以这种转化方法可以提升的空间很大。同时,该方法可用于探索其他用钙转和电转未成功的微生物。 Sepiolite-an inexpensive, resourceful, fibrous yet inoffensive mineral-made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4°C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.
作者 谭海东 王磊 林金涛 赵宗保
机构地区 中国科学院大连化学物理研究所生物技术部
出处 《生物工程学报》 CAS CSCD 北大核心 2010年第10期1379-1384,共6页 Chinese Journal of Biotechnology
基金 国家重点基础研究发展计划(973计划)(No2007CB707802)资助~~
关键词 矿石纳米纤维材料 海泡石 DNA转化 转化机制 mineral nanofibers sepiolite DNA transformation mechanism for transforming
分类号 Q78 [生物学—分子生物学]
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参考文献11

  • 1Yoshida N, Sato M. Plasmid uptake by bacteria: a comparison of methods and efficiencies. Appl Microbiol Biotechnol, 2009, 83(5): 791-798.
  • 2Yoshida N. Discovery and application of the Yoshida effect: nano-sized acicular materials enable penetration of bacterial cells by sliding friction force. Recent Pat Biotechnol, 2007, 1(3): 194-201.
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同被引文献27

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  • 2Zhang W, O' Connor K, Wang D I C, et al. Bioreduction with efficient recycling of NADPH by coupled permeabilized microorganisms [ J ]. Appl Environ Microbiol, 2009, 75 ( 3 ) : 687 -694.
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  • 4Medepalli K, Alphenam B W, Keynton R S, et al.A new technique for reversible permeabilization of live cells for intracellular delivery of quantum dots [ J ]. Nanotcchnology, 2013, 24 (20) :205101.
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  • 6Moran U,Phillips R, Milo R. SnapShot:key numbeS in biology [J].Cell,2010,141(7):1262-1262.
  • 7Yoshida N.Discovery and application of the Yoshida effect: nano- sized acicular materials enable penetration of bacterial cells by sliding friction force [ J ]. Recent Pat Bioteehnol, 2007, 1 ( 3 ) : 194-201.
  • 8Larkin J O, Casey G D, Tangney M, et ol. Effective tumor treatment using optimized ultrasound-mediated delivery of bleomycin [ J ].Ultrasound Med Biol, 2008,34 (3) : 406-413.
  • 9Sporty J L, Kabir M M, Turtehaub KW, et al. Single sample extraction protocol for the quantification of NAD and NADH redox states in Saccharomyces cerevisiae [ J ]. J Sep Sci, 2008,31 ( 18 ) : 3202-3211.
  • 10梁朝宁,薛燕芬,马延和.微生物降解利用木质纤维素的协同作用[J].生物工程学报,2010,26(10):1327-1332. 被引量：20

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生物工程学报
2010年 第10期

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