摘要
用浓度分别为０．０５、０．２５、１．０、５．０、１０．０、２０．０ｍｍｏｌ／Ｌ的ＳＡ溶液分别浸渍小麦幼苗地上部分（浸苗４５ｍｉｎ）和根部（浸根８ｈ），自然光下８ｈ后取第二展开叶测定。研究了外源水杨酸（ＳＡ）对叶片活性氧代谢的调节，探讨了ＳＡ抑制乙烯生物合成与活性氧之间可能的相互关系。结果表明，随着ＳＡ浓度增加，Ｈ２Ｏ２含量、超氧物歧化酶（ＳＯＤ）、酚特异性过氧化物酶（ＰＰＯＤ）活性呈先增加后降低的趋势，Ｏ２－产生速率则先降低后增加，过氧化氢酶（ＣＡＴ）活性逐渐降低，乙烯产生速率略有升高后迅速降低。小麦幼苗叶片活性氧代谢对ＳＡ浓度小于１．０ｍｍｏｌ／Ｌ的处理敏感。乙烯产生速率对外源ＳＡ敏感，体内Ｏ２－含量的增加不能引起乙烯产率上升。
The regulating effects of salicylic acid(SA)on the metabolism of active oxygen species(AOS) and the relationship between SA inhibiting ethylene biosynthesis and AOS metabolism in wheat leaves were studied.Wheat seedlings were treated by SA solution of different concentrations as follows:in one group the above ground parts of seedling were soaked separately in sets of SA solution for 45min ,in another group the root parts were soaked in SA solution.The second leaves of the two groups were measured after 8h under sunlight. The results showed that H 2O 2 contents,superoxide dismutase (SOD) and phenol specific peroxidase (PPOD)activities increased first and then decreased.Catalase(CAT) activities decreased apparently. Measurement of ethylene production rate showed that there was a significant decrease after slight increase.The results indicated that SA might be involved in the regulation of AOS metabolism,rate of ethylene production was sensitive to exogenous SA treatments,and increasing of O 2 - production rate in leaves could not raise the rate of ethylene production.
出处
《西南农业学报》
CSCD
北大核心
1999年第1期39-44,共6页
Southwest China Journal of Agricultural Sciences
关键词
水杨酸
小麦
活性氧
乙烯
salicylic acid
wheat
active oxygen species
ethylene
