摘要
目的观察环丙沙星的脂质体剂型(第3代氟喹诺酮类药物)对体外抗菌活性的影响。方法用硫酸铵梯度法方法,制备环丙沙星脂质体;用微量肉汤稀释法测定2种不同剂型(游离或脂质体包裹)对敏感和耐药铜绿假单胞菌的最低抑菌浓度(MIC),并描记杀菌曲线。结果与游离环丙沙星相比,环丙沙星脂质体对铜绿假单胞菌的MIC50与MIC90为前者的1/2和1/4,累计抑菌曲线和杀菌曲线左移。结论相对游离剂型,环丙沙星脂质体对铜绿假单胞菌有更强抗菌活性。
Objective To investigate liposomal ciprofloxacin antibacteri- al activity against Pseudomonas aeruginosa in vitro. Methods Ammoni- um sulfate gradients method was used to create liposomal ciprofloxacin. Broth dilution method was used to measure the MIC of free or liposomal ciprofloxacin against sensitive or resistant Pseudomonas aeruginosa, and killing curve was recorded. Results Compared with free form, MIC50 and MIC90 of liposomal ciprofloxin against Pseudomonas aeruginosa was down to 1/2 or 1/4 and accumulative bacteriostasis curve and killing curve shift left. Conclusion Liposomal ciprofloxin has stronger antibacterial activity against Pseudomonas aeruginosa than free ciprofloxin.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2010年第10期740-742,共3页
The Chinese Journal of Clinical Pharmacology
关键词
脂质体
环丙沙星
铜绿假单胞菌
liposomes
ciprofloxacin
Pseudomonas aeruginosa
