摘要
通过对３个玉米骨干自交系特异ＲＡＰＤ标记的克隆、测序、合成特异引物及ＳＣＡＲ－ＰＣＲ反应条件优化，成功地把其中一个自交系８１１２的特异ＲＡＰＤ标记（０．９ｋｂ）转换成了稳定的ＳＣＡＲ标记。
Three unique RAPD markers of 3 maize elite inbred lines were cloned and sequenced separately. Pairs of SCAR markers were designed and synthesized according to the 5 terminal nucleotide sequences there. Optimizing SCAR PCR reaction conditions for each inbred line were carried out. Only the unique RAPD marker in inbred line 8112 was successfully conversed into the SCAR marker. It may provide a new way for the application of RAPD marker to germplasm identification and crop seed purity testing.
出处
《高技术通讯》
EI
CAS
CSCD
1999年第3期45-47,共3页
Chinese High Technology Letters
基金
国家"九五"攻关资助项目
