摘要
用盆栽实验和室内分析相结合的方法,研究了Cu、Cr单一和复合污染对土壤酶活性的影响,旨在为土壤重金属复合污染的生物酶学评价提供参考依据。结果表明,在相同污染水平下,除Cr最低浓度(Cr5)处理对土壤过氧化氢酶有激活作用外,其余各浓度的Cu、Cr单一和复合污染均对供试的土壤脲酶、碱性磷酸酶、过氧化氢酶和硝酸还原酶活性产生抑制作用。4种酶相比较,Cu、Cr复合污染对过氧化氢酶活性抑制最小,而对土壤硝酸还原酶活性抑制最大。各处理有作物种植的土壤脲酶和碱性磷酸酶活性抑制率均大于相应的无作物种植处理,而土壤过氧化氢酶和硝酸还原酶的抑制率却小于无作物对照。有作物处理的4种供试酶活性抑制率与对应的无作物处理间均存在显著差异(P<0.01)。无论是否有作物种植,Cu、Cr复合污染对土壤脲酶、碱性磷酸酶活性抑制率产生不同程度的协同作用,对土壤过氧化氢酶有一定的拮抗作用。无作物种植时,Cu、Cr复合污染对土壤的硝酸还原酶活性抑制率为协同作用,而有作物种植时则为拮抗作用。建议以土壤脲酶和碱性磷酸酶活性来共同表征Cu、Cr复合污染毒害作用的大小。
Pot experiments and laboratory analysis were carried out to study Cu,Cr single and combined pollution influence on soil enzyme activities.The aim was to provide theoretical basis for biological enzyme evaluation of heavy metal cation-anion co-contamination in soil.The results showed that the same pollution level Cu and Cr singly or combined had inhibited effects on activity of urease,alkaline phosphatase,catalase and nitrate reductase.while in the 5 mg.kg-1 Cr treatment,the activity of catalase was promoted.Catalase was the least sensitive en-zyme to Cu-Cr cocontamination,while soil nitrate reductase was the most sensitive one to Cu-Cr cocontamination.Inhibition rates of urease and alkaline phosphatase activities in the planted treatment were larger than the non-crop control treatment.However,inhibition rate of cata-lase and nitrate reductase activities in the planted treatment were smaller than thoes in the non-crop control treatment.There was extremely significant difference in the planted treatment of all enzymes,compared with the non-plant treatment.Whether having plants or not,Cu and Cr combined pollution showed a certain synergyon on the inhibition rate of soil urease and alkaline phosphatase activities,a certain antago-nism on the inhibition of soil catalase activity,and a certain synergyon on nitrate reductase activity in non-crop control treatment,futhermore there was a certain antagonism on nitrate reductase activity in the planted treatment.Urease and alkaline phosphatase activities were suggest-ed to characterize the degree of Cu and Cr combined pollution in soil.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2010年第10期1971-1978,共8页
Journal of Agro-Environment Science
基金
国家支撑计划项目(2008BADA4B09)
西北农林科技大学"创新团队建设计划"
关键词
复合污染
CU
CR
酶活性
抑制率
co-contamination
Cu
Cr
enzyme activities
inhibition rate
