摘要
目的分析血浆EB病毒(Epstein-Barr virus,EBV)DNA定量测定对鼻咽癌监测复发及转移的价值。方法采用荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)技术,对42例鼻咽癌放疗后缓解者及28例鼻咽癌放疗后局部复发者、8例远处转移者血浆EBV-DNA进行定性及定量分析。结果鼻咽癌患者放疗后局部复发、转移者(阳性率75.0%;复制量中位数32 170.0copies/ml)亦高于放疗后缓解者。结论血浆EBV-DNA定量分析对鼻咽癌监测复发或转移很有意义,可作为鼻咽癌放疗后监测复发及转移的有效指标。
Objective To analyze quantitative determination of EB virus DNA in plasma on monitoring the recurrence of NPC and metastasis.Methods Using fluorescence quantitative polymerase chain reaction,comparisons were made between the 42 patients with nasopharyngeal carcinoma after radiotherapy and 28 local recurrences after radiotherapy,and 8 distant metastases.Results There were more patients prone to local recurrence and metastasis after radiotherapy（positive rate 75.0%,27/36 patients;the median of copy quantity 32 170.0copies/ml） than patients were relieved after radiotherapy.Conclusion Quantitative analysis of plasma EBV-DNA is effective in monitoring the recurrence or metastasis of nasopharyngeal carcinoma,so it can be adopted as the effective index to monitor nasopharyngeal carcinoma recurrence and metastasis.
出处
《湖北民族学院学报（医学版）》
2010年第3期20-21,共2页
Journal of Hubei Minzu University(Medical Edition)
基金
湖北省恩施州科技局指导性项目(No:恩州科业[2009]3号)
关键词
鼻咽癌
荧光定量PCR
血浆
EBV-DNA
nasopharyngeal carcinoma
fluorescent quantitative PCR
plasma
EBV-DNA
