摘要
目的评价BerEP4、E-cadherin及Calretinin在胸腹水液基细胞制片中对转移性腺癌和增生间皮细胞的鉴别诊断价值。方法选择180例胸腹水标本,其中腺癌150例、增生间皮30例,进行液基细胞制片免疫细胞化学染色,评价BerEP4、E-cadherin及Calretinin的鉴别诊断效果。结果 BerEP4和E-cadherin诊断转移性腺癌的敏感性分别为90.0%和88.0%,特异性均为100%。BerEP4和E-cadherin联合应用诊断胸腹水转移性腺癌的阳性率为97.3%。Calretinin对间皮细胞的敏感性为86.7%,特异性为100%。结论应用免疫细胞化学方法,BerEP4、E-cadherin及Calretinin能有效地鉴别胸腹水中腺癌细胞和增生间皮细胞,是有效的抗体组合,进一步提高了诊断的准确性。
Objective To evaluate the diagnostic value of BerEP4,E-cadherin and Calretinin in differentiating metastatic adenocarcinoma cells from mesothelial cells in pleuroperitoneal fluid.Methods The immunocytochemistry was used to detect the expression of BerEP4,E-cadherin and Calretinin antibodies after ThinPrep in 180 cases of pleuroperitoneal fluids,including 150 cases of metastatic adenocarcinoma and 30 cases of mesothelial cells.Results The sensitivities of BerEP4 and E-cadherin for metastatic adenocarcinomas were 90.0% and 88.0%,respectively,and both the specifities were 100%.The positive rate of metastatic adenocarcinomas was 97.3%（146/150）using combined detection of BerEP4 and E-cadherin.The sensitivity of calretinin for mesothelial cells was 86.7%,and the specifity was 100%.Conclusion The combined detection of BerEP4,E-cadherin and Calretinin is a very useful antibody panel in differential diagnosis for metastatic adenocarcinoma and mesothelial cells in pleuroperitoneal fluid,and could increase the diagnostic accuracy.
出处
《山西医科大学学报》
CAS
2010年第10期899-902,共4页
Journal of Shanxi Medical University
基金
西安医学院附属医院科研基金资助项目(xyfy07-10)
关键词
胸腹水
腺癌
免疫细胞化学
pleuroperitoneal fluid
adenocarcinoma
immunocytochemistry
