摘要
用过氧化物酶-抗过氧化物酶(PAP)法,对中华大蟾蜍消化道(冬眠期与非冬眠期),脑及其他组织的5-HT分布进行了研究。5-HT免疫染色细胞位于脑干中缝核区和间脑的第Ⅲ脑室腹侧的室管膜细胞区。阳性神经元呈圆形或卵圆形,细胞常有突起与其他阳性细胞突起相连,上述部位中还有一些阳性神经纤维。消化道的免疫染色细胞密度在胃幽门、胃体和胃贲门处最高,食道和十二指肠次之,大肠和小肠最低。非冬眠期蟾蜍消化道内免疫染色细胞密度明显高于冬眠期的(P<0.05)。阳性细胞位于粘膜上皮或腺上皮细胞间,细胞有一个或一个以上呈阳性反应的突起,有的突起伸入肠腔面或腺腔面,有的穿过基膜到达固有层,表明这些细胞兼有内、外分泌的功能。在甲状旁腺的主细胞间,肺呼吸性细支气管上皮和肺泡管上皮细胞间都有5-HT免疫染色细胞,细胞呈立方形、圆形、卵圆形或不规则形,常有几个细胞成簇分布。
The distribution of 5-hydroxytrypta mine (5-HT) immunostaining cells in the digestive tracts (hibernation and nonhibernation), the brain and other various tissues of Bufo bufo gargarizans was studied by peroxdase anti-peroxdase immunocytochemical method. In the brain, 5 HT immunostaining cells were localized in the raphen uelear area of brain stem and in the ependyma cell area of the ventriculus tertius of diencephalon. These immunostaining cells were round or oval. The cells usually possess processes which were filled with immunoreactive substance. Some of the processes were contact with the processes of other cells. A few 5-HT positive reactive nerve fibers were observed in the brain stem and the diencephalon.The density of 5-HT immunostaining cells in the digestive tubes were the highest in the pylorus, fundus, cardia of gaster, and moderate in the esophagus and duodenum and the lowest in the large intestine and the small intestine. The density of 5-HT immunostaining cells in the digestive tubes were higher in nonhibernant toads than in hibcrnant toads, By the statistical method, the difference of the density betwcen the two sorts of toads were notable (P<0.05). The 5-HT immunostaining cells were visualized to distribute between the epithelum cells of the mucosa or the epithelum cells of gland. These positive cells usually had one or more processes which contained 5-HT immunoreactive substance. Some were reached into lumen surface of the gland or intestine. Some were extended into lamina propria throuth the basal membrane. These results indicate that the 5-HT immunostaining cells in digestive tubes could release 5-HT by both endocrine and excrine ways.5-HT immunostaining cells were also localized in the area of the chief ceils of the parathyroid, the epithelum cells of the respiratory branchiol duct and the alveolar duct of lung.
出处
《实验生物学报》
CSCD
1990年第3期285-291,共7页
Acta Biologiae Experimentalis Sinica
基金
国家教委基金重点科学技术研究项目
关键词
中华大蟾蜍
血清素
免疫染色细胞
5-HT Immunostaining cells. Digestive tracts. Brain. Parathyroid. Lung.
