摘要
为初步调查SPF鸡感染鹦鹉热嗜性衣原体状况及相关SPF鸡胚源疫苗是否出现污染,本试验通过采集不同日龄的SPF鸡血清70份、SPF种蛋卵黄膜30份,收集市场上销售的SPF鸡胚源疫苗共41支,利用国产间接血凝试剂盒检测抗体,进口免疫荧光试剂盒分别测定其抗体、抗原阳性率,以评价SPF鸡鹦鹉热嗜性衣原体的流行状况和相关疫苗的污染状况。本试验结果显示,SPF鸡血清阳性率分别为31.4%(荧光法)、5.7%(间接血凝法);SPF种蛋阳性率33.3%,SPF鸡胚源疫苗平均阳性率31.7%。SPF鸡已经感染了鹦鹉热嗜性衣原体,且发现经鸡胚卵黄膜而传播病原的新途径,进而造成SPF鸡胚源疫苗出现衣原体污染。因此,加强SPF鸡鹦鹉热嗜性衣原体监测已势在必行。
The preliminary investigation is to explore whether SPF chicken are infected by avian Chlamydophila psittaci（C.psittaci）,then whether the associated avian vaccines contaminated with C.psittaci.In current study,70 serums from different ages of SPF chicken were collected to detect the antibody against C.psittaci using commercial indirect haemagglutination（IHA） test and immunofluorescence assay（IFA）.Meanwhile,30 egg yolk membranes were chosen for detecting the antigen presence by IMAGENTM Chlamydia kits.Moreover,41 batches of vaccines originated from SPF eggs were collected from local distributors in four provinces and chlamydial contamination was confirmed by IFA test kits.The seropositivity was 31.4%（IFA） and 5.7%（IHA） in 70 serum samples,respectively.The positive of antigen was 33.3% in SPF eggs and 31.7% in chicken vaccines,respectively.The antigen was identified in the egg yolk membrane.The above evidences indicated that Chlamydophila psittaci was prevalent in SPF chicken and it was vertical transmit way from the yolk membranes of SPF eggs to offspring as well as contamination for chicken vaccines.It is urgent to take some concreted measures to monitor the infection of Chlamydophila psittaci in SPF chicken.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第10期166-169,共4页
China Animal Husbandry & Veterinary Medicine
基金
教育部博士点基金项目(200902010510749)
北京市共建项目(200902910510772)
