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钻井废弃液中石油污染物及细菌生态多样性

Petroleum Pollutants and Bacteria Diversity in Waste Drilling Fluid
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摘要 采用GC/MS分析方法,发现平湖油田钻井废弃液中含有烃类、酸类和酯类等23种有机污染物质,含量达到49 800 mg/L。运用16S rRNA基因分析法测得的微生物生态多样性表明,废弃液中微生物种类丰富,包括γ和β变形杆菌,γ-Proteobacteria是优势菌群,占克隆总数78.6%。Oceanospirillales、Pseudomonadales和Enterobacteriales是γ-Proteobacteria纲的主要菌群。 The organic contaminations and the bacterial diversity were investigated in the waste drilling fluid from Pinghu Oilfield by using GC/MS. The result showed that there were 23 kinds of organic pollutants containing hydrocarbons, acids and esters in the waste drilling fluid. The bacterial 16S rRNA gene clone library was constructed to detect the community diversity in the waste drilling fluid. The phylogenetic analysis indicated that most of clones were clustered in β, γ-Proteobacteria with the phylotypes belonging to γ-Proteobacteria accounting for 78.6% of the clone library. Oceanospirillales, Pseudomonadales and Enterobacteriales were the main appearances in γ-Proteobacteria.
出处 《城市环境与城市生态》 CAS CSCD 2010年第5期42-44,共3页 Urban Environment & Urban Ecology
基金 国家自然科学基金(50574040) 上海市科委资助项目(071607014)
关键词 钻井废弃液 16SRRNA基因 细菌多样性 waste drilling fluid 16S rRNA gene bacterial diversity
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