摘要
目的 探讨血管紧张素Ⅱ的1型受体拮抗剂缬沙坦对兔心肌梗死后室性心律失常发生的影响及其可能机制.方法 24只家兔随机（随机数字法）分为假手术组、心肌梗死组和缬沙坦组.三组均在无菌条件下开胸,其中心肌梗死组和缬沙坦组分别结扎左冠状动脉前降支.缬沙坦组术后第2天给予缬沙坦（10mg·kg-1·d-1）灌胃,三组均喂养12周.三组家兔分别在心肌梗死前、心肌梗死12周后记录程序刺激诱发的室性心动过速/室颤（VT/VF）发生次数,并采用全细胞膜片钳技术记录L型钙通道电流（Ica-L）的变化.结果 心肌梗死12周后,缬沙坦组较心肌梗死组明显降低VT/VF的发生次数（3.2±0.6 vs.11.7±1.8,P＜0.05）;假手术组、心肌梗死组和缬沙坦组Ica-L电流密度分别为（-6.29±0.65）pA/pF,（-9.12±0.73）pA/pF和（-6.75±0.64）pA/pF,心肌梗死组显著高于假手术组及缬沙坦组（P＜0.05）;缬沙坦组与假手术组比较无统计学意义（P＞0.05）.结论 缬沙坦明显降低家兔心肌梗死后室性心动过速/室颤的发生,其机制可能与抑制家兔心肌梗死后Ica-L有关.
Objective To determine the effects of valsartan, a specific angiotensin Ⅱ type 1 receptor blockade, on arrhythmia in rabbits after myocardial infarction and to discuss the mechanism. Method Twentyfour rabbits were randomly （random number） divided into sham operated （SO） group （ n = 8）, myocardial infarction （MI） group （ n = 8） and valsartan （VAL） group （ n = 8）. The rabbits of SO group were operated upon with median stemotomy without left ventricular coronary artery hgature. The rabbits of MI group and VAL group had median stemotomy with left ventricular coronary artery ligature. After MI, the rabbits of VAL group were fed with border zone of infracted left ventricular wall and the L-type calcium current was recorded by whole-cell patch clamp technique. Results Ventricular tachycardia or fibrillation （VT/VF） episodes were markedly decreased in VAL group than that in MI group [（3.2 ± 0. 6） vs. （ 11.7 ± 1.8）] after 12 weeks. The density of Ica-L current was higher in MI group than that in SO group and VAL group [（ - 9.12 ± 0.73） pA/pF vs. （ - 6.29 ± 0.65） pA/pF and （ - 6.75 ± 0.64） pA/pF], （ P ＜ 0.05）, however, there were no significant differences in Ica-L current between So group and VAL group （ P ＞ 0.05）. Conclusions Valsartan reduces the VT/VF episodes in rabbits after MI. The effects of valsartan may be attributed to the inhibited electrical remodeling after MI.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2010年第9期949-952,共4页
Chinese Journal of Emergency Medicine
关键词
心肌梗死
室性心律失常
L型钙通道电流
膜片钳
缬沙坦
兔
Myocardial infarction
Ventricular arrhythmias
L-type calcium current
Patch clamp
Valsartan
Rabbit
