慢性乙型肝炎患者HBV前C区及BCP区变异与血清细胞因子的关系被引量：2

The relationship between HBV precore and basal core promoter mutations and serum cytokines in patients with chronic hepatitis B

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摘要目的探讨乙型肝炎e抗原(HBeAg)阴性和阳性慢性乙型肝炎(CHB)患者乙型肝炎病毒(HBV)前C区、基本核心启动子(BCP)区变异特点以及与血清细胞因子干扰素(IFN)-γ、白细胞介素(IL)-10水平的关系。方法将120例HBV DNA阳性CHB患者(HBeAg阴性和阳性各60例)与60例健康体检者(对照组)纳入研究。荧光定量聚合酶链反应(PCR)法检测HBeAg阴性和阳性组患者HBV DNA水平,直接测序法检测两组前C区G1896A变异及BCP区A1762T和G1764A变异,双抗夹心酶联免疫吸附试验检测血清细胞因子IFN-γ/、IL-10的水平。结果 120例HBV DNA阳性CHB患者HBV前C区和BCP区变异总检出率为60.00%(72/120),其中HBeAg阴性组变异检出率为80.00%(48/60),HBeAg阳性组变异检出率为40.00%(24/60),两组比较,差异有显著性(x^2=20.00,P=0.000)。HBeAg阴性组G1896A变异(38.33%)和联合变异(G1896A、A1762T和G1764A同时变异,25.00%)的检出率明显高于HBeAg阳性组(16.67%、0.00%)(分别x^2=7.06,P=0.008;x^2=17.14,P=0.000)。变异组血清IFN-7水平为(102.33±27.20)pg/mL,明显高于无变异组(79.18±16.43)pg/mL及对照组(35.77±4.23)pg/mL(分别t=5.72,t=19.33,均P=0.000);变异组血清IL-10水平为(28.13±7.00)pg/mL,明显高于无变异组(13.91±5.42)pg/mL及对照组(13.68±2.27)pg/mL(分别t=12.50,t=15.65,均P=0.000)。结论 G1896A变异和联合变异更常见于HBeAg阴性CHB;G1896A和A1762T/G1764A变异与血清细胞因子IFN-γ和IL-10水平升高有关。 Objective To study the characteristics of hepatitis B virus （HBV） preeore G1896A mutation and basal core promoter （BCP） A1762T and G1764A mutations among HBeAg-negative and HBeAg-positive chronic hepatitis B （CHB） patients, and to explore the relationship between the above mutations and the serum levels of interferon-7 （IFN-γ） and interleukin-10 （IL-10） in these patients. Methods 120 patients with HBV DNA positive CHB including 60 HBeAg-negative CHB （group A） and 60 HBeAg-positive CHB （group B）, and 60 healthy persons were enrolled in this study. The serum HBV DNA of group A and B were determined by real-time fluorescence quantitative polymerase chain reaction （PCR）. The HBV precore G1896A mutation and BCP A1762T and G1764A mutation in group A and B were detected by PCR and direct sequencing. The levels of IFN-γ and IL-10 in serum were measured by enzyme linked immunosorbent assay. Results The total incidence of preeore and BCP mutations was 60. 00% （72/120） in 120 patients, the incidence of precore and BCP mutation in group A and B was 80. 00% （48/60） and 40. 00% （24/60） respectively （x^2 = 20. 00,P = 0. 000）. The incidence of precore G1896A mutation （38. 33%） and the united mutation （G1896A and A1762T and G1764A mutation, 25.00%） in group A were significantly higher than those in group B （16. 67 %, 0. 00 %）（x^2 = 7. 06, P = 0. 008; x^2 = 17. 14, P = 0. 000）. The serum level of IFN-γ in the mutation group were（102. 33 ± 27. 20）pg/mL, which were significantly higher than （79. 18 ± 16. 43）pg/mL in the non-mutation group （t = 5.72,P = 0. 000） and （35.77 ± 4. 23）pg/mL in the healthy control group （t = 19. 33,P = 0. 000） . The serum level of IL-10 in the mutation group were（28. 13 ± 7. 00）pg/mL , which were also significant ly higher than（ 13.91 ± 5.42）pg/mL in the non-mutation group（t = 12. 50, P = 0. 000） and （13.68 ± 2. 27）pg/mL in the healthy control group （t = 15.65,P = 0. 000）. Conclusion The G1896A mutation and the united mutation commonly occur in the HBeAg negative CHB patients. The mutations of G1896A and A1762T/G1764A may be related to the serum increased levels of IFN- γ and IL-10.

作者蒋孝华李小芬

机构地区南华大学附属第一医院

出处《中国感染控制杂志》 CAS 2010年第5期320-323,共4页 Chinese Journal of Infection Control

关键词肝炎乙型慢性肝炎病毒乙型前核心区基本核心启动子基因变异干扰素-Γ 白细胞介素-10 hepatitis B, chronic hepatitis B virus preeore basal core promoter gene mutation interferon-γ interleukin-10

分类号 R512.62 [医药卫生—内科学]

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