摘要
目的从高山被孢霉中克隆的Δ6-脂肪酸脱氢酶基因与植物表达载体pBA121连接,构建重组质粒pBMACL6,采用农杆菌介导的大豆子叶节转化系统成功地将该基因导入到栽培大豆吉林47品种中,获得一批转基因植株。方法利用子叶节外植体诱导出丛生芽和再生植株,利用卡那霉素的抗性筛选培养基连续筛选以及PCR方法、DNA分子杂交分析检测转基因植株中的Δ6-脂肪酸脱氢酶基因表达状况。结果经PCR检测和DNA分子杂交分析,初步证明Δ6-脂肪酸脱氢酶基因已导入并整合到大豆基因组中。结论成功地将Δ6-脂肪酸脱氢酶基因导入并整合到大豆基因组中。
Objective To introduce Δ6-fatty acid desaturase genes from Mortieralla alpina into jilin47 via Agrobacterium infection method,adentitious,and obtain transgenic plants. Methods Adentitious buds and regenerated plants were induced from cotyledon nodes and screened continuously in kanamycin resistant culture medium. PCR and Southern blot were used to detect expression of the Δ^6-fatty acid desaturase genes in transgenic plants. Results PCR and Southern blot analysis from transgenic plants proved that the Δ6-fatty acid desaturase genes were transferred and integrated into soybean genome. Conclusion The Δ6-fatty acid desaturase genes are transferred and integrated into soybean genome successfully.
出处
《成都医学院学报》
CAS
2010年第2期93-96,共4页
Journal of Chengdu Medical College
基金
国家自然科学基金资助项目(编号:30200176)
关键词
Δ6-脂肪酸脱氢酶基因
大豆
农杆菌介导转化
转基因植株
Δ6-fatty acid desaturase gene
Soybean （Glycin max L）
Agrobacterium-mediated transformation
transgenic plant
