摘要
目的：建立聚合酶链反应技术（ＰＣＲ）检测我国性病高危人群分泌物标本中的Ｍｇ－ＤＮＡ，以探讨Ｍｇ感染在性传播疾病中的作用及地位。方法：用ＰＣＲ技术检测来自昆明、广东、上海、南京等地性病门诊病人４２３例、性罪错者２６１例尿道分泌物或宫颈分泌物的Ｍｇ－ＤＮＡ，并与正常对照组进行了比较。结果：①性病门诊组４２３例标本的Ｍｇ－ＰＣＲ阳性率达１５．１％（６４／６２３），性罪错组的Ｍｇ－ＰＣＲ阳性率为８．８１％（２３／２６１），均与正常对照组（１．３７％，１／７３）差异极显著（Ｐ＜０．０１）；②来自广东、昆明地区的性病门诊标本的Ｍｇ－ＰＣＲ阳性率为２０．１％（２６／１２９）及１８．８％（２６／１３８），均与南京、上海地区的性病Ｍｇ－ＰＣＲ阳性率有显著差异（Ｐ＜０．０５）；③３１例无ＮＧＨ，ＵＵ，ＣＴ感染标本的Ｍｇ－ＰＣＲ阳性率为１２．９％（４／３１），与有三种不同病原体单独或混合感染组的Ｍｇ－ＰＣＲ阳性率差异不显著（Ｐ＞０．０５）。结论：①我国性病高危人群即性病门诊及性罪错人群有着较高的Ｍｇ感染率；②我国性病高发地区如广东、昆明等地有着较高的Ｍｇ感染率；③Ｍｇ可以不依赖于任何病原微生物而存在于人的泌尿生殖道中，而且可能是?
Objectives: Creating polymerase chain reaction(PCR)
technique to detect Mycoplasma genitalium(Mg) infection rates in sexually transmitted
disease(STD) high risk populations from different geographical areas in our country,discussing
the role of Mg in STD. Methods: The urogenital specimens from 423 cases of STD
department,261 sexually promiscuous population and 73 normal persons,who were from
Nanjing,Shanghai,Guangdong and Kunmin,were detected for MgDNA by polymerase chain
reaction technique. Results: MgDNAPCR positive rate in control group was 1.79%,which was
significantly lower than that(15.1%) in group of STD department and that(8.81%) in group of
promiscuous population(P<0.01),respectively.Otherwhise,there was a remarkable difference of
MgDNAPCR positive rate between groups of STD department and promiscuous population.Mg
infection rate of STD population in Guangdong and Kunmin was much higher than that of
Shanghai,Nanjing(P<0.05) and not differences was found between Shanghai and Nanjing,also
Gua dong and Kunmin.There were not differences between group without NGH,UU,CT infection
and groups with NGH,UU,CT infection alone or commonly(P>0.05). Conclusions: Mg infection
rate was higher in STD highrisk populations(including STD patients and promiscuous persons)
than that in normal group in China.Higher MgDNA positive rate was found in Guangdong and
Kunmin,where there was a higher STD occurance.There was a same MgDNA positive rate in the
specimens with or without other microorganism infection.Mg might also be an important
microorganism of STD,which is helpful for Mg epidemiologic observation and controlling the
transmission of STD.
出处
《男科学报》
CSCD
1999年第2期88-90,共3页
关键词
生殖支原体
聚合酶链反应
性病
Mycoplasma genitaliumSexually transmitted
diseasesPolymerase chain reactionng
