摘要
目的探讨柯萨奇病毒Ｂ１（ＣＶＢ１）感染与多发性肌炎（ＰＭ）发病的关系，利用ＣＶＢ１接种豚鼠建立ＰＭ模型。方法分别用０．１ｍｌ（Ａ组）和０．３ｍｌ（Ｂ组）毒力（ＴＣＩＤ５０，即５０％细胞感染剂量）为１０－５的ＣＶＢ１分别一次性接种２４只和３６只豚鼠，并用３ｍｇ（阈下剂量）兔肌匀浆加完全弗氏佐剂每周免疫１次；另有１０只豚鼠，仅用３ｍｇ兔肌匀浆加完全弗氏佐剂每周免疫１次为对照组（Ｃ组），共５周。结果Ａ、Ｂ组３周后均有豚鼠出现四肢肌无力等症状，肌酶谱明显升高，豚鼠股肌病理呈局灶性炎性改变，Ｃ组无异常。Ａ、Ｂ组５周后的肌酶谱升高阳性率存在显著差异（Ａ组为１１．８％，Ｂ组为３８．２％）。结论ＣＶＢ１感染是ＰＭ发病诱因之一，其毒力及接种量与ＰＭ的发病相关。
Objective To study the relation between infection with coxackievirus
B1(CVB1) and onset of polymysitis in the polymyositis models of guinea pigs infected with
CVB1. Method Sixty guinea pigs were innoculated with 0.1 ml of CVB1 in group A and with 0.3
ml of CVB1 in group B. The CVB1 toxicity being 10-5 TCID50. Then they were immunized once a
week with 3 mg rabbit muscle homogenates in Freunds complete adjuvant, ten guinea pigs
immunized once a week only with 3 mg rabbit muscle homogenates in Freunds complete
adjuvant as a control (group C). Results Some guinea pigs innoculated with CVB1 showed
muscular weakness of their limbs, the serum enzymes were evidently elevated and the
histopathological, muscular changes of polymyositis were confirmed 3 weeks after the viral
infection, the guinea pigs of group C were normal. The positive rates of elevation in muscle
enzymes were significantly different between the two groups of guinea pigs. In group A
elevation in muscle enzymes was found in 11.8%, whereas in group B it appeared in 38.2% five
weeks after the viral infection. Conclusion Not only infection with coxsackievirus B1 could
induce PM but also its quantity and toxicity were correlated with the onset of polymyositis.
出处
《中华神经科杂志》
CAS
CSCD
1999年第3期146-148,共3页
Chinese Journal of Neurology
基金
江苏省教委自然科学基金
