摘要
目的研究在动脉粥样硬化发病过程中内源性一氧化碳的合成酶血红素氧化酶－１ｍＲＮＡ和蛋白表达的变化规律。方法通过原位杂交、免疫组化染色等指标观察动脉粥样硬化发病过程中内源性一氧化碳的合成酶血红素氧化酶－１基因及其蛋白的变化规律。结果１发现动脉粥样硬化的发展过程中主动脉及冠状动脉壁的血红素氧化酶－１ｍＲＮＡ表达有逐渐增高的趋势。并且血红素氧化酶－１ｍＲＮＡ主要分布于主动脉和冠状动脉内皮细胞。２发现在动脉粥样硬化的发展过程中血红素氧化酶－１蛋白在主动脉和冠状动脉壁的表达也有逐渐增高的趋势。并且血红素氧化酶－１免疫组化主要在主动脉和冠状动脉的内皮细胞呈阳性。结论内源性一氧化碳的合成酶血红素氧化酶－１ｍＲＮＡ及其蛋白表达在动脉粥样硬化发病过程中呈逐渐增高的趋势。
Objective To research the changes in expression of heme oxygenase-1(carbon monoxide synthase) mRNA and protein during atherosclerosis development. Methods In situ hybridixation, immunohistcohernistry stain were performed. Results 1. the positive score of heme oxygenase-1 mRNA is distributed in endothelial cell of aorta and coronary artery,the expression of heme oxygenase 1 mRNA on aorta and coronary artery raises in atherosclerosis development.2. the positive score of heme oxygenase-1 portein is distributed in endothelial cell of aorta and coronary artery,the expression of heme oxygenase-1 portein on aorta and coronary artery raises in atherosclerosis development. Conclusions The expression of carbon monoxide synthase heme oxygenase-1 mRNA and protein raises in atherosclerosis development.
出处
《中国微循环》
1999年第2期85-87,共3页
Journal of Chinese Microcirculation
